Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area
Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area
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DOI：
10.1016/j.vetpar.2008.08.015
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发表时间：
2008-11-25
影响因子：
2.6
通讯作者：
de la Fuente, Jose
中科院分区：
文献类型：
--
作者：
Alamzan, Consuelo;Medrano, Citlaly;de la Fuente, Jose
边缘无原体是一种由蜱传播的牛病原体，在世界范围内引起牛无原体病。主要表面蛋白（MSPs）参与宿主-病原体和蜱-病原体的相互作用，并被用作边缘拟南芥菌株遗传特征的标记。A.边缘基因型在世界各地的流行地区是高度可变的。在无形体病暴发期间，边缘单胞杆菌菌株的遗传组成仅在一项研究中得到表征，该研究报告了受感染牛的单一msp1 α基因型。然而，需要更多的信息来确定是否单一基因型导致无形体病暴发，或者是否多种基因型可在流行地区的单一牛群中引起未出生牛的疾病。本研究的目的是表征墨西哥塔毛利帕斯州爆发的牛无形体病爆发的边缘A.菌株的遗传多样性。利用msp4和msp1 α基因序列在分子水平上对A. marginate基因型进行了鉴定。结果表明，在急性牛无形体病暴发期间，牛中存在几种边缘螺旋体基因型，这表明机械传播或通过同样有效的独立传播事件的随机生物传播可能解释了流行地区急性牛无形体病暴发期间牛群中边缘螺旋体基因型的频率。本研究结果证实了世界范围内边缘单胞菌在流行地区的遗传异质性。无形体病控制措施的制定和实施取决于对流行地区边缘单胞虫流行病学的了解，包括对引起牛无形体病暴发的菌株遗传多样性的描述。(C) 2008 Elsevier B.V.版权所有
Anaplasma marginate is a tick-borne pathogen of cattle that causes the disease bovine anaplasmosis worldwide. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and have been used as markers for the genetic characterization of A. marginate strains. A. marginate genotypes are highly variable in endemic areas worldwide. The genetic composition of A. marginate strains during anaplasmosis outbreaks has been characterized in one study only which reported a single msp1 alpha genotype in infected cattle. However, more information is required to characterize whether a single genotype is responsible for an anaplasmosis outbreak or whether multiple genotypes can cause disease in naive cattle within a single herd in endemic areas. The aim of this study was to characterize the genetic diversity of A. marginate strains from an outbreak of bovine anaplasmosis in the State of Tamaulipas, Mexico. A. marginate genotypes were characterized at the molecular level using msp4 and msp1 alpha gene sequences. The results revealed that several A. marginate genotypes are present in cattle during acute anaplasmosis outbreaks, thus suggesting that mechanical transmission or stochastic biological transmission through equally efficient independent transmission events may explain A. marginate genotype frequency in a cattle herd during acute bovine anaplasmosis outbreaks in endemic areas. The results reported herein corroborated the genetic heterogeneity of A. marginate strains in endemic regions worldwide. The development and implementation of anaplasmosis control measures is dependent upon understanding the epidemiology of A. marginate in endemic regions, including the characterization of the genetic diversity of strains that produce outbreaks of bovine anaplasmosis. (C) 2008 Elsevier B.V. All rights reserved.