A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster.
A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster.
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DOI：
10.1007/s10616-005-5414-3
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发表时间：
2005-09-01
期刊：
影响因子：
2.2
通讯作者：
Ueda, H
中科院分区：
文献类型：
--
作者：
Kawasaki, H;Hirose, S;Ueda, H
核抽提物的制备是生化鉴定转录因子等核因子的关键步骤。我们建立了一种从果蝇预蛹或蛹中制备核提取物的新方法。这种方法很简单，特别适用于从难以大量获得的材料中进行小规模分离，例如被蛹壳覆盖的特定组织或动物身体。
Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.