Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics
Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics
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DOI：
10.1016/j.febslet.2012.09.043
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发表时间：
2012-11-16
期刊：
影响因子：
3.5
通讯作者：
Hazeki, Osamu
中科院分区：
文献类型：
--
作者：
Hazeki, Kaoru;Nigorikawa, Kiyomi;Hazeki, Osamu
PtdIns（3）P（phosphatidylinositol 3-phosphate）是吞噬体成熟的重要信号分子。Vps 34在吞噬体PtdIns（3）P的产生中起主要作用。然而，新生成的PtdIns（3）P的命运还没有得到很好的描述。在这里，我们表明，在缺乏PTEN或PIKfyve的RAW 264.7巨噬细胞中，从吞噬体膜消除PtdIns（3）P显著延迟。在用PIKfyve抑制剂处理的PTEN缺陷细胞中，PtdIns（3）P的降解几乎丧失，表明PTEN和PIKfyve是吞噬体PtdIns（3）P代谢中的两个主要参与者。(c)2012年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
PtdIns(3) P (phosphatidylinositol 3-phosphate) is a signaling molecule important for phagosome maturation. The major role of Vps34 in production of phagosomal PtdIns(3) P has been indicated. However, the fate of the newly generated PtdIns(3) P has not been well described. Here we show that elimination of PtdIns(3) P from phagosomal membrane was significantly delayed in RAW264.7 macrophages lacking PTEN or PIKfyve. In the PTEN-deficient cells treated with a PIKfyve inhibitor, degradation of PtdIns(3) P was almost lost, indicating that PTEN and PIKfyve are two major players in phagosomal PtdIns(3) P metabolism. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.