Distinct Aurora B pools at the inner centromere and kinetochore have different contributions to meiotic and mitotic chromosome segregation.
Distinct Aurora B pools at the inner centromere and kinetochore have different contributions to meiotic and mitotic chromosome segregation.
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DOI：
10.1091/mbc.e23-01-0014
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发表时间：
2023-05-01
影响因子：
3.3
通讯作者：
Lacefield, Soni
中科院分区：
文献类型：
--
作者：
Cairo, Gisela;Greiwe, Cora;Jung, Gyu Ik;Blengini, Cecilia;Schindler, Karen;Lacefield, Soni
正确的染色体分离依赖于双向运动染色体-微管连接的建立，这通常需要多轮的释放和再连接。极光B和C激酶磷酸化动粒蛋白以释放无张力附着。多种途径将极光B/C募集到着丝粒和动粒。我们研究了这些途径如何有助于后期开始的时间和纠正的着丝粒微管附件在芽殖酵母减数分裂和有丝分裂。我们发现Bub 1/Bub 3途径定位的池以不同的方式设定了减数分裂和有丝分裂的正常持续时间。我们的减数分裂数据表明，该途径的中断导致PP 1动粒定位，这去磷酸化Cdc 20过早后期发病的模型。对于纠错，Bub 1/Bub 3和COMA途径在减数分裂中是单独重要的，但在有丝分裂中是补偿性的。最后，我们发现haspin和Bub 1/3通路共同发挥作用，以确保在小鼠卵子发生错误的纠正。我们的研究结果表明，每个招聘途径定位空间上不同的kinetochorse定位极光B/C池，功能不同的减数分裂和有丝分裂之间。
Proper chromosome segregation depends on the establishment of bioriented kinetochore–microtubule attachments, which often requires multiple rounds of release and reattachment. Aurora B and C kinases phosphorylate kinetochore proteins to release tensionless attachments. Multiple pathways recruit Aurora B/C to the centromere and kinetochore. We studied how these pathways contribute to anaphase onset timing and correction of kinetochore–microtubule attachments in budding yeast meiosis and mitosis. We find that the pool localized by the Bub1/Bub3 pathway sets the normal duration of meiosis and mitosis, in differing ways. Our meiosis data suggests a model that disruption of this pathway leads to PP1 kinetochore localization, which dephosphorylates Cdc20 for premature anaphase onset. For error correction, the Bub1/Bub3 and COMA pathways are individually important in meiosis but compensatory in mitosis. Finally, we find that the haspin and Bub1/3 pathways function together to ensure error correction in mouse oogenesis. Our results suggest that each recruitment pathway localizes spatially distinct kinetochore-localized Aurora B/C pools that function differently between meiosis and mitosis.
影响因子：
5.5
作者：
Galli M;Diani L;Quadri R;Nespoli A;Galati E;Panigada D;Plevani P;Muzi-Falconi M
通讯作者：
Muzi-Falconi M
Aurora激酶B调节小鼠卵母细胞中的染色体比对。
影响因子：
2.5
作者：
Shuda, Kristy;Schindler, Karen;Ma, Jun;Schultz, Richard M.;Donovan, Peter J.
通讯作者：
Donovan, Peter J.
DOI：
10.3791/64580
发表时间：
2022-10-11
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Cairo G;MacKenzie A;Tsuchiya D;Lacefield S
通讯作者：
Lacefield S