A GmSIN1/GmNCED3s/GmRbohBs Feed-Forward Loop Acts as a Signal Amplifier That Regulates Root Growth in Soybean Exposed to Salt Stress
A GmSIN1/GmNCED3s/GmRbohBs Feed-Forward Loop Acts as a Signal Amplifier That Regulates Root Growth in Soybean Exposed to Salt Stress
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GmSIN1/GmNCED3s/GmRbohBs 前馈环路充当信号放大器，调节暴露于盐胁迫的大豆的根系生长
DOI：
10.1105/tpc.18.00662
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发表时间：
2019-09-01
期刊：
影响因子：
11.6
通讯作者：
Xiang, Fengning
中科院分区：
文献类型：
--
作者：
Li, Shuo;Wang, Nan;Xiang, Fengning
脱落酸（阿坝）和活性氧（ROS）是植物响应盐胁迫的关键信号分子，然而，这些信号是如何转导和放大的仍不清楚。在这里，大豆（Glycine max）盐诱导NAM/ATAF 1/2/CUC 2（NAC）转录因子编码的盐诱导的NAC 1（GmSIN 1）被证明是这一过程的关键组成部分。过量表达GmSIN 1促进大豆根系生长和耐盐性，提高盐胁迫下的产量; RNA干扰介导的GmSIN 1敲低则具有相反的效果。GmSIN 1对盐胁迫的快速响应需要阿坝和ROS的参与，GmSIN 1对根伸长和耐盐性的影响是通过提高细胞内阿坝和ROS含量来实现的。GmSIN 1通过在迄今为止尚未描述的位点与大豆中的9-顺式-环氧类胡萝卜素双加氧酶编码基因（GmNCED 3 s，与阿坝合成相关）和大豆中的呼吸爆发氧化酶同源物B基因（GmRbohBs，与ROS产生相关）的启动子结合而上调它们。GmSIN 1、GmNCED 3s和GmRbohBs共同构成了一个正反馈系统，能够快速积累阿坝和ROS，有效地放大初始盐胁迫信号。这些结果表明阿坝和ROS含量的联合调节提高了大豆的耐盐性。
Abscisic acid (ABA) and reactive oxygen species (ROS) act as key signaling molecules in the plant response to salt stress; however, how these signals are transduced and amplified remains unclear. Here, a soybean (Glycine max) salinity-induced NAM/ATAF1/2/CUC2 (NAC) transcription factor encoded by SALT INDUCED NAC1 (GmSIN1) was shown to be a key component of this process. Overexpression of GmSIN1 in soybean promoted root growth and salt tolerance and increased yield under salt stress; RNA interference-mediated knockdown of GmSIN1 had the opposite effect. The rapid induction of GmSIN1 in response to salinity required ABA and ROS, and the effect of GmSIN1 on root elongation and salt tolerance was achieved by boosting cellular ABA and ROS contents. GmSIN1 upregulated 9-cis-epoxycarotenoid dioxygenase coding genes in soybean (GmNCED3s, associated with ABA synthesis) and Respiratory burst oxidase homolog B genes in soybean (GmRbohBs, associated with ROS generation) by binding to their promoters at a site that has not been described to date. Together, GmSIN1, GmNCED3s, and GmRbohBs constitute a positive feed-forward system that enables the rapid accumulation of ABA and ROS, effectively amplifying the initial salt stress signal. These findings suggest that the combined modulation of ABA and ROS contents enhances soybean salt tolerance.