FAHFA footprint in the visceral fat of mice across their lifespan
FAHFA footprint in the visceral fat of mice across their lifespan
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小鼠一生内脏脂肪中的 FAHFA 足迹
DOI：
10.1016/j.bbalip.2020.158639
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Feng Yu-Qi
中科院分区：
文献类型：
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作者：
Zhu Quan-Fei;Yan Jing-Wen;Ni Jian;Feng Yu-Qi
羟基脂肪酸的脂肪酸酯 (FAHFA) 是一类新型内源性脂质，具有抗炎和抗糖尿病作用，具有治疗 2 型糖尿病 (T2D) 的潜力。鉴于FAHFA对T2D等年龄相关疾病的重要调节和治疗作用，我们假设它们也可能在生长、发育和衰老过程中发挥关键作用。在这里，我们利用化学同位素标记辅助液相色谱-质谱法研究了小鼠整个生命周期内脏脂肪组织（VAT）中的 FAHFA 足迹，以获得了解 FAHFA 在生长、发育和衰老中的作用的潜在线索。 VAT 样本是从 9 个不同年龄（1、2、3、6、9、12、15、18 和 24 个月）的 80 只 C57BL/6J 雄性小鼠中采集的。结果显示，各年龄段小鼠的VAT中共检测到51个FAHFA家族，包括301个区域异构体，且VAT中的FAHFA（包括家族和区域异构体）数量随着年龄的增长而增加，从1月龄小鼠的35个家族（186±0个区域异构体）增加到18月龄小鼠的46个家族（278±6个区域异构体）。此外，小鼠每 100 mg VAT 中 12 个 FAHFA 家族的含量与年龄高度相关，且在中年期（3-15 个月）通常较低。然而，由于中年小鼠的 VAT 质量比年轻或年长的小鼠高 4-5 倍，因此中年小鼠中 VAT 中大多数 FAHFA 区域异构体的总量有所增加。据我们所知，这是第一项研究表明 51 个 FAHFA 家族的区域异构体数量和 15 个 FAHFA 家族的丰度强烈依赖于年龄，这将有助于理解 FAHFA 对生长、发育和衰老影响的机制。
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with anti-inflammatory and anti-diabetic effects, having the potential to treat type 2 diabetes (T2D). In view of the important regulatory and therapeutic actions of FAHFAs on age-related diseases such as T2D, we hypothesized that they may also play crucial roles in the growth, development, and aging process. Here, we investigated the FAHFA footprint in the visceral adipose tissue (VAT) of mice across lifespan to attain potential clues for understanding the roles of FAHFAs in growth, development, and aging using chemical isotope labeling assisted liquid chromatography-mass spectrometry. VAT samples were harvested from 80 C57BL/6J male mice of nine different ages (1, 2, 3, 6, 9, 12, 15, 18, and 24 months). The results showed that a total of 51 FAHFA families, including 301 regioisomers, were detected in the VAT of mice of all ages, and the number of FAHFAs (both family and regioisomer) in VAT increased with age, from 35 families (186 ± 0 regioisomers) at 1-month-old mice to 46 families (278 ± 6 regioisomers) in 18-month-old mice. Furthermore, the content of 12 FAHFA families per 100 mg of VAT of mice was highly correlated with age, and was usually low in the middle-age (3–15 months). However, because the VAT mass was 4–5 fold higher in middle-aged mice compared to younger or older mice, the total amount of most of the FAHFA regioisomers in VAT was increased in middle-aged mice. To the best of our knowledge, this is the first study to show that the number of regioisomers from 51 FAHFA families and abundance of 15 FAHFA families are strongly dependent on age, which would be helpful for understanding the mechanisms underlying the effects of FAHFAs on growth, development, and aging.