Molecular Epidemiology of Outbreak-Associated and Wild-Waterfowl-Derived Newcastle Disease Virus Strains in Finland, Including a Novel Class I Genotype
Molecular Epidemiology of Outbreak-Associated and Wild-Waterfowl-Derived Newcastle Disease Virus Strains in Finland, Including a Novel Class I Genotype
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DOI：
10.1128/jcm.01427-12
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发表时间：
2012-11-01
影响因子：
9.4
通讯作者：
Huovilainen, Anita
中科院分区：
文献类型：
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作者：
Lindh, Erika;Ek-Kommonen, Christine;Huovilainen, Anita
新城疫（ND）是由新城疫病毒（NDV；或禽副粘病毒-1）致病株引起的一种高度传染性的严重家禽疾病。新城疫是全世界野生鸟类的地方病，也是经济上最重要的家禽病原体之一。大多数已发表的毒株都是与暴发相关的毒株，而野生鸟类中出现的非致病性新城疫毒株能够转化为毒性更强的形式，对家禽构成持续威胁，但研究仍然较少。 2006 年至 2010 年期间，我们对芬兰野生水禽的泄殖腔和口咽样本中的新城疫病毒 RNA 进行了筛查：715 只鸟类中有 39 只呈阳性（患病率 5.5%）。为了系统发育的目的，对 37 个菌株的部分或全长 F 基因进行了测序。我们还对芬兰三起新城疫病毒暴发的病毒进行了特征分析，并讨论了这些暴发相关毒株和野鸟相关毒株之间的关系。我们发现所有水禽NDV分离株都是I类或II类基因型I的弱毒株。我们还分离出一种遗传上不同的I类毒株（青色/芬兰/13111/2008），与1987年在北爱尔兰分离的HIECK87191毒株在系统发育上分组在一起。它们似乎共同形成了一种新的I类基因型，与其他已知的NDV在遗传上至少有12%的差异。
Newcastle disease (ND) is a highly contagious, severe disease of poultry caused by pathogenic strains of Newcastle disease virus (NDV; or avian paramyxovirus-1). NDV is endemic in wild birds worldwide and one of the economically most important poultry pathogens. Most of the published strains are outbreak-associated strains, while the apathogenic NDV strains that occur in wild birds, posing a constant threat to poultry with their capability to convert into more virulent forms, have remained less studied. We screened for NDV RNA in cloacal and oropharyngeal samples from wild waterfowl in Finland during the years 2006 to 2010: 39 of 715 birds were positive (prevalence, 5.5%). The partial or full-length F genes of 37 strains were sequenced for phylogenetic purposes. We also characterized viruses derived from three NDV outbreaks in Finland and discuss the relationships between these outbreak-associated and the wild-bird-associated strains. We found that all waterfowl NDV isolates were lentogenic strains of class I or class II genotype I. We also isolated a genetically distinct class I strain (teal/Finland/13111/2008) grouping phylogenetically together with only strain HIECK87191, isolated in Northern Ireland in 1987. Together they seem to form a novel class I genotype genetically differing from other known NDVs by at least 12%.