Phosphorylation of half and all sites in H+,K(+)-ATPase results in opposite changes in tryptophan fluorescence.
Phosphorylation of half and all sites in H+,K(+)-ATPase results in opposite changes in tryptophan fluorescence.
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H ,K( )-ATP酶中一半和全部位点的磷酸化导致色氨酸荧光的相反变化。
DOI：
10.1006/bbrc.1993.2248
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发表时间：
1993
影响因子：
3.1
通讯作者：
K. Taniguchi
中科院分区：
文献类型：
--
作者：
H. Eguchi;S. Kaya;K. Taniguchi
在猪胃H+，K（+）-ATP酶中，Trp荧光强度随ATP和乙酰磷酸（AcP）的磷酸化而增加，最大值分别为0.86和0.37%。由ATP形成的磷酸酶的最大量、由AcP形成的磷酸酶的最大量、由无机磷酸盐（Pi）形成的磷酸酶的最大量以及与酶结合的ATP的最大量的化学计量被示出为接近1：2：2：2。形成的磷酸酶被证明是翻转的。K+的加入使ATP的磷酸酶量减少到十分之一，但使AcP或Pi的磷酸酶量减少到一半。数据显示，Trp荧光随着一半位点的磷酸化而增加，但随着所有位点的磷酸化而降低。
The Trp fluorescence increased accompanying phosphorylation by ATP and Acetyl phosphate (AcP) in pig stomach H+,K(+)-ATPase, respectively, to maximum values of 0.86 and 0.37%. The stoichiometry of the maximum amount of phosphoenzyme formed from ATP, that from AcP, that from inorganic phosphate (Pi), and the maximum amount of ATP binding to the enzyme was shown to be close to 1:2:2:2. Phosphoenzymes formed were shown to be turning over. The addition of K+ reduced the amount of phosphoenzyme from ATP to one-tenth but reduced those from AcP or Pi to only the half. The data show that the Trp fluorescence increased with phosphorylation of half of the sites but decreased with phosphorylation of all sites.