Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa.
Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa.
复制标题
副性腺液对公牛附睾尾精子活力、获能和顶体反应的影响。
DOI：
10.1002/j.1939-4640.2000.tb02098.x
复制
发表时间：
2000
影响因子：
--
通讯作者：
Gary J. Killian
中科院分区：
文献类型：
--
作者：
A. Way;Lester C. Griel;Gary J. Killian
用同一头荷斯坦公牛（n = 6）的附睾尾精子和附性腺液（AGF）研究了AGF对精子活力和顶体完整性的影响。输精管的手术插管，使单独收集附睾尾流出物和AGF从每头公牛。附睾尾流出物与AGF从同一公牛收集或培养基单独孵育。共孵育后，将精子（5 x 10（7）个精子/mL）在单独的培养基中或在获能条件（10 μ g/mL肝素）下孵育16小时。每2小时，将一等份精子暴露于溶血磷脂酰胆碱（100 μ g/mL），以诱导获能精子的顶体反应。无论给药与否，精子活力均随时间推移而降低。总的来说，在AGF中孵育的精子比未在AGF中孵育的精子具有更少的顶体完整的活精子。与未在AGF中预孵育的精子相比，当精子暴露于AGF中时，随着时间的推移，存活率显著降低（P <0.05）。当精子未在AGF中预孵育时，暴露于肝素和溶血磷脂酰胆碱后，观察到显著更多（P <0.05）的顶体反应活精子。我们的结论是，暴露于AGF的精子加速细胞死亡，并从精浆中快速去除精子是至关重要的最大活力。
The effect of accessory sex gland fluid (AGF) on viability and acrosomal integrity of spermatozoa was examined with cauda epididymal spermatozoa and AGF from the same Holstein bull (n = 6). Surgical cannulation of the vasa deferentia enabled the separate collection of cauda epididymal effluent and AGF from each bull. Cauda epididymal effluent was incubated with either AGF collected from the same bull or medium alone. Following coincubation, spermatozoa (5 x 10(7) sperm/mL) were incubated in medium alone or under capacitating conditions (10 microg/mL heparin) for 16 hours. Every 2 hours, an aliquot of spermatozoa was exposed to lysophosphatidylcholine (100 microg/mL) to induce the acrosome reaction in capacitated spermatozoa. Sperm motility decreased over time regardless of treatment. Overall, spermatozoa incubated in AGF had fewer acrosome-intact live spermatozoa than did those not incubated in AGF. Viability was significantly (P < .05) compromised over time when spermatozoa were exposed to AGF, compared with those not preincubated in AGF. Significantly more (P < .05) acrosome-reacted live spermatozoa were seen following exposure to heparin and lysophosphatidylcholine when spermatozoa were not preincubated in AGF. We conclude that exposure of spermatozoa to AGF accelerates cell death and that rapid removal of spermatozoa from seminal plasma is critical for maximal viability.