Microcavity substrates casted from self-assembled microsphere monolayers for spheroid cell culture.
Microcavity substrates casted from self-assembled microsphere monolayers for spheroid cell culture.
复制标题
由自组装微球单层铸造的微腔基底，用于球状细胞培养。
DOI：
10.1007/s10544-014-9863-3
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发表时间：
2014-08
影响因子：
2.8
通讯作者：
Parekkadan, Biju
中科院分区：
文献类型：
--
作者：
Shen, Keyue;Lee, Jungwoo;Yarmush, Martin L.;Parekkadan, Biju
关键词：
多细胞球体是一种重要的三维细胞培养模型，它反映了体内微环境的许多关键方面。本文提出了一种可扩展的，自组装为基础的方法来制造微腔基板的多细胞球体细胞培养。疏水性玻璃微珠自组装成一个紧密堆积的单分子层通过表面张力，重力和横向毛细作用力的组合作用在水-空气界面的聚合物溶液。随后将填充的珠单层包埋在干燥的聚合物层中。该表面被用作复制具有完美球形形状的微腔基底的模板。我们证明了使用的基板在监测肿瘤球体的形成过程中，一个概念验证的放大制造程序到标准的微孔板格式，其应用程序在测试癌症药物反应的背景下，骨髓基质细胞。该技术为生物和药物筛选应用提供了一种简单有效的方法，即在不使用微加工设备的情况下形成高密度均匀尺寸的球状体。
Multicellular spheroids are an important 3-dimensional cell culture model that reflects many key aspects of in vivo microenvironments. This paper presents a scalable, self-assembly based approach for fabricating microcavity substrates for multicellular spheroid cell culture. Hydrophobic glass microbeads were self-assembled into a tightly packed monolayer through the combined actions of surface tension, gravity, and lateral capillary forces at the water-air interface of a polymer solution. The packed bead monolayer was subsequently embedded in the dried polymer layer. The surface was used as a template for replicating microcavity substrates with perfect spherical shapes. We demonstrated the use of the substrate in monitoring the formation process of tumor spheroids, a proof-of-concept scale-up fabrication procedure into standard microplate formats, and its application in testing cancer drug responses in the context of bone marrow stromal cells. The presented technique offers a simple and effective way of forming high-density uniformlysized spheroids without microfabrication equipment for biological and drug screening applications.
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