Recombinant mucin biotechnology and engineering.
Recombinant mucin biotechnology and engineering.
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DOI：
10.1016/j.addr.2022.114618
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发表时间：
2022-11
影响因子：
16.1
通讯作者：
Sangwook Park;Joe Chin-Hun Kuo;H. Reesink;M. Paszek
中科院分区：
文献类型：
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作者：
Sangwook Park;Joe Chin-Hun Kuo;H. Reesink;M. Paszek
粘蛋白代表了生物材料、治疗学和其他生物技术的一类基本上未开发的聚合物构件。因为粘蛋白聚合物骨架是遗传编码的，所以可以使用重组技术制造具有限定的物理和生物化学性质的序列特异性粘蛋白。粘蛋白的侧链O-聚糖越来越多地参与免疫调节、抑制病原体毒力和其他生物化学活性。工程化细胞生产系统的最新进展使具有精确调节的聚糖侧链的重组粘蛋白的可规模化合成成为可能，为调节基于粘蛋白的产品的生物功能提供了令人兴奋的可能性。新的代谢和化学酶策略使粘蛋白O-聚糖的进一步调整和功能化成为可能，为扩大粘蛋白结构单元的化学多样性和功能开辟了新的可能性。在这篇综述中，我们讨论了这些进展，以及工程粘蛋白在生物医学应用中的机会，从体外模型到治疗。
Mucins represent a largely untapped class of polymeric building block for biomaterials, therapeutics, and other biotechnology. Because the mucin polymer backbone is genetically encoded, sequence-specific mucins with defined physical and biochemical properties can be fabricated using recombinant technologies. The pendent O-glycans of mucins are increasingly implicated in immunomodulation, suppression of pathogen virulence, and other biochemical activities. Recent advances in engineered cell production systems are enabling the scalable synthesis of recombinant mucins with precisely tuned glycan side chains, offering exciting possibilities to tune the biological functionality of mucin-based products. New metabolic and chemoenzymatic strategies enable further tuning and functionalization of mucin O-glycans, opening new possibilities to expand the chemical diversity and functionality of mucin building blocks. In this review, we discuss these advances, and the opportunities for engineered mucins in biomedical applications ranging fromin vitromodels to therapeutics.