EFFECT OF PENTOXIFYLLINE ON TISSUE-INJURY AND PLATELET-ACTIVATING-FACTOR PRODUCTION DURING ISCHEMIA-REPERFUSION INJURY
EFFECT OF PENTOXIFYLLINE ON TISSUE-INJURY AND PLATELET-ACTIVATING-FACTOR PRODUCTION DURING ISCHEMIA-REPERFUSION INJURY
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DOI：
10.1016/s0741-5214(05)80005-9
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发表时间：
1995-05-01
影响因子：
4.3
通讯作者：
SILVER, D
中科院分区：
文献类型：
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作者：
ADAMS, JG;DHAR, A;SILVER, D
目的：己酮可可碱减轻与缺血再灌注损伤相关的代谢紊乱。本研究评价己酮可可碱对骨骼肌缺血再灌注损伤过程中血小板激活因子（PAE）产生和组织损伤的影响。方法：采用离体犬股薄肌模型。第 1 组肌肉进行 5 小时缺血和 20 小时再灌注（n = 10）；第 2 组肌肉在再灌注前 10 分钟接受己酮可可碱 15 mg/kg 全身输注（n = 6）；第3组肌肉在再灌注前10分钟接受己酮可可碱25 mg/kg全身输注（rt = 6）。通过闪烁邻近测定法从肌肉静脉流出物中测量 PAF。通过活体染色和面积测定法评估肌肉损伤。结果：与第 1 组相比，第 2 组的 PAF 水平在再灌注 10、15 和 30 分钟时有所下降，但未达到显着性。与第 1 组相比，第 3 组的 PAP 水平在再灌注的所有时间均下降，但仅在再灌注 10 分钟时才达到显着性 (p < 0.05)。三组之间的肌肉重量没有显着差异。第 1 组 (77.26% +/- 20.38%) 和第 2 组 (60.49% +/- 23.97%) 之间没有观察到肌肉坏死程度的差异 (p = 0.08)；与第 1 组相比，第 3 组的肌肉坏死程度显着降低 (44.55% +/- 21.47%) (p < 0.05)。 结论：在缺血性骨骼肌再灌注前给予 25 mg/kg 己酮可可碱可显着降低肌肉坏死的程度，并在再灌注的所有时间段静脉流出液中的 PAP 水平显着降低（在10 分钟）。这些结果表明己酮可可碱可能通过在再灌注关键时期抑制 PAP 的产生来减少缺血再灌注的组织损伤。
Purpose: Pentoxifylline lessens the metabolic derangements associated with ischemia-reperfusion injury. This study evaluated the effects of pentoxifylline on platelet-activating factor (PAE) production and tissue injury during skeletal muscle ischemia-reperfusion injury.Methods: The isolated canine gracilis muscle model was used. Group 1 muscles were subjected to 5 hours of ischemia and 20 hours of reperfusion (n = 10); group 2 muscles received pentoxifylline, 15 mg/kg, systemic infusion 10 minutes before reperfusion (n = 6); group 3 muscles received pentoxifylline, 25 mg/kg, systemic infusion 10 minutes before reperfusion (rt = 6). PAF was measured from muscle venous effluent by the scintillation proximity assay method. Muscle injury was assessed by vital staining and planimetry.Results: PAF levels in group 2 were decreased at 10, 15, and 30 minutes of reperfusion compared with group 1 but did not reach significance. PAP levels in group 3 were decreased at all times of reperfusion compared with group 1 but attained significance only at 10 minutes of reperfusion (p < 0.05). No significant differences in muscle weight were noted among the three groups. No differences in the extent of muscle necrosis was observed between group 1 (77.26% +/- 20.38%) and group 2 (60.49% +/- 23.97%) (p = 0.08); there was a significant reduction in the extent of muscle necrosis in group 3 (44.55% +/- 21.47%) compared with group 1 (p < 0.05).Conclusions: The administration of pentoxifylline at 25 mg/kg before reperfusion of ischemic skeletal muscle decreased significantly the extent of muscle necrosis and PAP levels in the venous effluents at all times of reperfusion (significantly at 10 minutes). These results suggest that pentoxifylline may decrease tissue injury of ischemia-reperfusion by inhibiting the production of PAP during critical periods of reperfusion.