A NEW DROSOPHILA HOMEO BOX GENE IS EXPRESSED IN MESODERMAL PRECURSOR CELLS OF DISTINCT MUSCLES DURING EMBRYOGENESIS
A NEW DROSOPHILA HOMEO BOX GENE IS EXPRESSED IN MESODERMAL PRECURSOR CELLS OF DISTINCT MUSCLES DURING EMBRYOGENESIS
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DOI：
10.1101/gad.4.12a.2098
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发表时间：
1990-12-01
影响因子：
10.5
通讯作者：
FRASCH, M
中科院分区：
文献类型：
--
作者：
DOHRMANN, C;AZPIAZU, N;FRASCH, M
一些果蝇同源框基因已被证明可以控制胚胎特定位置或细胞群的细胞命运。由于涉及复杂内脏器官（例如肌肉组织和神经系统）模式形成的机制仍然很大程度上未知，因此我们试图识别和分析在这些组织中特异性表达的新同源框基因。在此，描述了一种含有同源框和 PRD 重复序列的基因的分子分析和表达模式。该基因被命名为S59，在少量节段重复的中胚层细胞中表达。原肠胚形成后2小时。逐渐地，四组表达 S59 的中胚层细胞出现在每个腹部半节中，每组在与周围的成肌细胞融合后产生特定的体肌。因此，特定肌肉的个体前体细胞（我们称之为“创始人细胞”）在中胚层发育过程中相对较早地被指定。这些细胞中特定同源框基因的表达表明，在种带伸长后，不同的基因表达程序在中胚层细胞亚群中活跃，从而导致其发育命运的规范。除了中胚层之外，S59还在中枢神经系统神经元细胞及其前体细胞的子集中以及中肠小区域的细胞中表达。
Several Drosophila homeo box genes have been shown to control cell fates in specific positions or cell groups of the embryo. Because the mechanisms involved in the pattern formation of complex internal organs, such as the musculature and the nervous sytem, are still largely unknown, we sought to identify and analyze new homeo box genes specifically expressed in these tissues. Here, the molecular analysis and expression pattern of one such gene, containing both a homeo box and a PRD repeat, is described. This gene, designated S59, is expressed in a small number of segmentally repeated mesodermal cells .apprx. 2 hr postgastrulation. Gradually, four groups of S59-expressing mesodermal cells appear in each abdominal hemisegment, each one giving rise to a particular somatic muscle after fusion with surrounding myoblasts. Thus, individual precursors for particular muscles, which we call "founder cells", are specified relatively early during mesodermal development. The expression of a particular homeo box gene in these cells suggests that distinct programs of gene expression are active in subsets of mesodermal cells after germ band elongation, resulting in a specification of their developmental fates. In addition to the mesoderm, S59 is expressed in a subset of neuronal cells of the CNS and their precursors and also in cells of a small region of the midgut.