Genome-wide analysis of ovate family proteins in Arabidopsis.
Genome-wide analysis of ovate family proteins in Arabidopsis.
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DOI：
10.1016/s1006-8104(13)60022-7
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Chang, Y.
中科院分区：
文献类型：
--
作者：
Huang JianPing, Huang JianPing;Li HongLing, Li HongLing;Chang, Y.
拟南芥卵形家族蛋白(AtOFP)是一个新发现的与拟南芥TALE(3-AA环延伸同源结构域蛋白)同源结构域蛋白相互作用的植物特异蛋白家族。在此，基于生物信息学分析，我们发现拟南芥基因组实际上编码了17个含有卵形结构域的蛋白。其中一种名为AtOFP19，此前尚未被确认。根据其氨基酸序列的相似性，AtOFP蛋白可分为两类。大多数AtOFP定位于细胞核，其中4个定位于叶绿体，其余2个定位于细胞质。基于全基因组微阵列的基因表达分析涉及47个营养和生殖发育阶段，揭示了AtOFP具有不同的表达模式。蛋白质相互作用的研究表明，9个AtOFP只与同源同源结构域蛋白相互作用，而同源同源结构域蛋白是植物分生组织功能和叶片发育的基本调节因子。我们的工作可能会为卵子家族蛋白的功能基因组学研究提供重要的线索，这可能涉及到以前未知的植物发育控制机制。
Arabidopsis thaliana ovate family proteins (AtOFPs) is a newly found plant-specific protein family interacting with TALE (3-aa loop extension homeodomain proteins) homeodomain proteins in Arabidopsis. Here, based on bioinformatic analysis, we found that Arabidopsis genome actually encoded 17 OVATE domain-containing proteins. One of them, AtOFP19, has not been previously identified. Based on their amino acid sequence similarity, AtOFPs proteins can be divided into two groups. Most of the AtOFPs were located in nuclear, four of them were presented in chloroplast and the remaining two members appeared in cytoplasmic. A genome-wide microarray based gene expression analysis involving 47 stages of vegetative and reproductive development revealed that AtOFPs have diverse expression patterns. Investigation of proteins interaction showed that nine AtOFPs only interacted with TALE homeodomain proteins, which are fundamental regulators of plant meristem function and leaf development. Our work could provide important leads toward functional genomics studies of ovate family proteins, which may be involved in a previously unrecognized control mechanism in plant development.