Molecular evolution of H6 influenza viruses from poultry in southeastern China: Prevalence of H6N1 influenza viruses possessing seven A/Hong Kong/156/97 (H5N1)-like genes in poultry
Molecular evolution of H6 influenza viruses from poultry in southeastern China: Prevalence of H6N1 influenza viruses possessing seven A/Hong Kong/156/97 (H5N1)-like genes in poultry
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DOI：
10.1128/jvi.76.2.507-516.2002
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发表时间：
2002-01-01
影响因子：
5.4
通讯作者：
Shortridge, KF
中科院分区：
文献类型：
--
作者：
Chin, PS;Hoffmann, E;Shortridge, KF
A/teal/Hong Kong/W312/97 （H6N1）流感病毒与人类H5N1和H9N2流感病毒具有相似的编码内部蛋白的基因，表明H6N1病毒可能成为新的人类病原体。对1975 ~ 1981年和1997 ~ 2000年分离的29株H6流感病毒进行了抗原和遗传分析，探讨了H6流感病毒的分子流行病学和进化特征。根据其抗原性特征确定了两个不同的群体。系统进化分析表明，1999年和2000年从陆生家禽分离的H6N1病毒均与A/teal/Hong Kong/W312/97 （H6N1）亲缘关系密切，且与A/Hong Kong/156/97 （H5N1）的核苷酸序列同源性超过96%。1999年和2000年陆生病毒的血凝素（HA）：在HAI到HA2的切割位点没有多个碱性氨基酸；然而，在RAI的144位和145位（H3编号）之间发现了一个独特的天冬氨酸插入。这些陆生H6N1病毒的神经氨酸酶缺失了a /Hong Kong/156/97 （H5N1）特有的19个氨基酸。进化分析表明，这些H6N1病毒与A/鹌鹑/香港/ g1 /97样H9N2病毒共同进化，更适应陆生家禽。1999年和2000年陆生A/teal/Hong Kong/W312/97 （H6N1）样病毒，连同H9N2病毒，可能参与了1997年致病性H5N1流感病毒的起源。香港家禽市场发现含有A/Hong Kong/156/97 （H5N1）病毒8个基因中的7个的H6N1病毒，这引发了以下与流感大流行防范有关的基本问题：致病性H5N1病毒是否会再次出现，以及H6N1病毒是否会直接跨越物种屏障传播给哺乳动物？
The A/teal/Hong Kong/W312/97 (H6N1) influenza virus and the human H5N1 and H9N2 influenza viruses possess similar genes encoding internal proteins, suggesting that H6N1 viruses could become novel human pathogens. The molecular epidemiology and evolution of H6 influenza viruses were characterized by antigenic and genetic analyses of 29 H6 influenza viruses isolated from 1975 to 1981 and 1997 to 2000. Two distinct groups were identified on the basis of their antigenic characteristics. Phylogenetic analysis revealed that all H6N1 viruses isolated from terrestrial poultry in 1999 and 2000 are closely related to A/teal/Hong Kong/W312/97 (H6N1), and the nucleotide sequences of these viruses and of A/Hong Kong/156/97 (H5N1) were more than 96% homologous. The hemagglutinin (HA) of the 1999 and 2000 terrestrial viruses does: not have multiple basic amino acids at the site of cleavage, of HAI to HA2; however, a unique insertion of aspartic acid in RAI between positions 144 and 145 (H3 numbering) was found. The neuraminidase of these terrestrial H6N1 viruses has a deletion of 19 amino acids characteristic of A/Hong Kong/156/97 (H5N1). Evolutionary analysis suggested that these H6N1 viruses coevolved with A/quail/Hong Kong/G1/97-like H9N2 viruses and became more adapted to terrestrial poultry. These terrestrial 1999 and 2000 A/teal/Hong Kong/W312/97 (H6N1)-like viruses, along with the H9N2 viruses, could have been involved in the, genesis of the pathogenic H5N1 influenza viruses of 1997. The presence of H6N1 viruses in poultry markets in Hong Kong that possess seven of the eight genes of the A/Hong Kong/156/97 (H5N1) virus raises the following fundamental questions relevant to influenza pandemic preparedness: could the pathogenic H5N1 virus reemerge and could the H6N1 viruses directly cross the species barrier to mammals?.