A comparison study between polymeric ligand and monomeric ligand for oligopeptide adsorption.
A comparison study between polymeric ligand and monomeric ligand for oligopeptide adsorption.
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DOI：
10.1016/j.jcis.2010.12.045
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发表时间：
2011-03
影响因子：
9.9
通讯作者：
Jing Feng;Li-Xue Fu;Jun Wang;Wen Wang;Jihong Li;Yitao Qiao;P. Sun;Zhi Yuan
中科院分区：
文献类型：
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作者：
Jing Feng;Li-Xue Fu;Jun Wang;Wen Wang;Jihong Li;Yitao Qiao;P. Sun;Zhi Yuan
这项工作旨在通过研究它们对寡肽的吸附亲和力、容量和选择性来比较两种类型的亲和配体，即聚合配体和单体配体。选择肽 NH2-VVRGCTWW-COOH (VW-8) 作为目标吸附物，而分别选择组氨酸 (His)、天冬氨酸 (Asp) 和亮氨酸 (Leu) 作为配体。对于每种配体，分别将单体（M）和聚合（P）形式引入Sepharose基质上，得到相应的吸附剂。使用等温滴定量热法 (ITC) 的亲和力测试和使用静态吸附实验的吸附能力均表明，具有聚合物配体的吸附剂 (MX-P) 对 VW-8 表现出比具有单体配体的吸附剂 (MX-M) 更好的吸附能力。特别是，MX-PHis的亲和常数为2.39×106M−1，对VW-8的吸附容量为77.4mg/g，比MX-MHis高约8-10倍。基于核磁共振（NMR）和ITC数据解释了聚合物配体和单体配体之间这种不同的吸附能力，结果表明聚合物配体的这种更好的特性归因于它们良好的柔韧性，这促进了协同效应以及配体与肽的可及性。此外，选择性吸附实验表明，所有具有聚合物配体的吸附剂对肽VW-8都表现出良好的选择性。
This work aimed to compare two types of affinity ligands, i.e. polymeric and monomeric ligands, by investigating their adsorption affinity, capacity and selectivity to oligopeptide. The peptide NH2-VVRGCTWW-COOH (VW-8) was chosen as the target adsorbate, while histidine (His), aspartic acid (Asp), and leucine (Leu) were selected as the ligands, respectively. For each kind of ligand, both monomeric (M) and polymeric (P) forms were introduced onto the Sepharose matrix respectively to obtain the corresponding adsorbents. Both affinity tests using isothermal titration calorimetry (ITC) and adsorption capacities using static adsorption experiments indicated that the adsorbents with polymeric ligands (MX-P) exhibited better adsorption ability for VW-8 than the adsorbents with monomeric ligands (MX-M). In particular, the MX-PHis exhibited its affinity constant of 2.39×106M−1and its adsorption capacity of 77.4mg/g for VW-8, which was approximately 8–10 times higher than that of MX-MHis. Such distinct adsorption abilities between polymeric and monomeric ligands were interpreted based on nuclear magnetic resonance (NMR) and ITC data, and the results indicated that such better characters of polymeric ligands were ascribed to their good flexibility which facilitated the cooperative effects as well as the accessibility of ligands to the peptide. Additionally, the selective adsorption experiments indicated that all the adsorbents with polymeric ligands exhibited good selectivity to the peptide VW-8.