Fluorescence molecular tomography resolves protease activity in vivo
Fluorescence molecular tomography resolves protease activity in vivo
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DOI：
10.1038/nm729
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发表时间：
2002-07-01
期刊：
影响因子：
82.9
通讯作者：
Weissleder, Ralph
中科院分区：
文献类型：
--
作者：
Ntziachristos, Vasilis;Tung, Ching-Hsuan;Weissleder, Ralph
目前正在系统地努力开发新技术，以实现体内完整生物体的分子传感。使用近红外荧光分子信标和考虑到组织中光子传播的扩散性质的反演技术，我们能够获得原位神经胶质瘤中蛋白酶的三维体内图像。我们证明，可以在深层组织中以高位置精度检测酶激活的荧光染料，可以解析不同信标对酶的分子特异性，并且信标激活的断层扫描与酶浓度线性相关。断层成像方法为研究生物功能提供了一系列新功能；例如，通过多光谱成像识别分子表达模式或持续监测治疗药物的功效。
Systematic efforts are under way to develop novel technologies that would allow molecular sensing in intact organisms in vivo. Using near-infrared fluorescent molecular beacons and inversion techniques that take into account the diffuse nature of photon propagation in tissue, we were able to obtain three-dimensional in vivo images of a protease in orthopic gliomas. We demonstrate that enzyme-activatable fluorochromes can be detected with high positional accuracy in deep tissues, that molecular specificities of different beacons towards enzymes can be resolved and that tomography of beacon activation is linearly related to enzyme concentration. The tomographic imaging method offers a range of new capabilities for studying biological function; for example, identifying molecular-expression patterns by multispectral imaging or continuously monitoring the efficacy of therapeutic drugs.