Identification of a novel gene, HaABRC5, from Helianthus annuus (Asteraceae) that is upregulated in response to drought, salinity, and abscisic acid
Identification of a novel gene, HaABRC5, from Helianthus annuus (Asteraceae) that is upregulated in response to drought, salinity, and abscisic acid
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DOI：
10.3732/ajb.91.2.184
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发表时间：
2004-02-01
影响因子：
3
通讯作者：
Baird, WV
中科院分区：
文献类型：
--
作者：
Liu, XN;Baird, WV
利用mRNA转录本的差异显示，我们获得了一个部分cDNA克隆，RSC 5-U，这是上调暴露于高盐度。然后通过cDNA末端的快速扩增克隆了一个更长的812个核苷酸的cDNA，命名为HaABRC 5。该全长cDNA含有423个核苷酸的开放阅读框，编码141个氨基酸，包括“二分核靶向序列”。“推导的氨基酸序列与数据库中的已知基因没有相似性。采用定量逆转录聚合酶链反应（RT-PCR）对HaABRC 5的表达进行了更详细的研究。HaABRC 5是上调干旱，高盐，外源性应用脱落酸（阿坝）。使用基因组末端的快速扩增克隆HaABRC 5上游的229个核苷酸的启动子序列。在HaABRC 5启动子区域内发现了三个ABA响应元件。因此，HaABRC 5可能是一种ABA反应核蛋白，在植物逆境应答中发挥作用。
Using differential display of mRNA transcripts, we obtained a partial cDNA clone, RSC5-U, that is upregulated by exposure to high salinity. A longer cDNA of 812 nucleotides, designated HaABRC5, was then cloned by rapid amplification of cDNA ends. This full-length cDNA contains an open reading frame of 423 nucleotides encoding 141 amino acids, including a "bipartite nuclear targeting sequence." The deduced amino acid sequence had no similarity to known genes in the database. The expression of HaABRC5 was investigated in more detail using quantitative reverse transcriptase-polymerise chain reaction. HaABRC5 is upregulated by drought, high salinity, and exogenous application of abscisic acid (ABA). The promoter sequence of 229 nucleotides, upstream of HaABRC5, was cloned using rapid amplification of genomic ends. Three ABA-responsive elements were found within the HaABRC5 promoter region. Therefore, HaABRC5 is probably an ABA-responsive nuclear protein playing a role in plant stress response.