Development of SM5-1-conjugated ultrasmall superparamagnetic iron oxide nanoparticles for hepatoma detection
Development of SM5-1-conjugated ultrasmall superparamagnetic iron oxide nanoparticles for hepatoma detection
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DOI：
10.1016/j.bbrc.2008.06.126
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发表时间：
2008-09-19
影响因子：
3.1
通讯作者：
Guo, Yajun
中科院分区：
文献类型：
--
作者：
Kou, Geng;Wang, Shuhui;Guo, Yajun
超顺磁性氧化铁（USPIO）纳米颗粒是目前在磁共振成像（MRI）中用作造影剂的磁铁矿或磁铁矿纳米颗粒。本研究将共沉淀的USPIO与人源化的SM5-1抗体偶联，制备了一种靶向造影剂SM-USPIO，该抗体能与人肝癌细胞发生特异性反应。流式细胞术和共聚焦显微镜证实了SM-USPIO与HCC细胞系ch-hep-3的结合和内化。此外，SM-USPIO被证明能够选择性地积聚在肿瘤细胞中，导致MRI t2加权信号强度显著降低。生物分布研究表明SM-USPIO在裸鼠ch-hep-3肿瘤中有效积累。在ch-hep-3瘤裸鼠体内的研究表明，SM-USPIO作为造影剂的MRI具有良好的诊断能力，提示SM-USPIO有潜力成为HCC诊断的靶向造影剂。(C) 2008爱思唯尔公司版权所有。
Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are maghemite or magnetite nanoparticles currently used as contrast agent in magnetic resonance imaging (MRI). In this study, a targeted contrast agent (SM-USPIO) was prepared by conjugating coprecipitated USPIO to a humanized SM5-1 antibody which can specifically react with human hepatocellular carcinoma (HCC) cells. The binding and internalization of SM-USPIO to the HCC cell line ch-hep-3 was confirmed by flow cytometry and confocal microscopy. Furthermore, SM-USPIO was demonstrated to be able to selectively accumulate in the tumor cells, resulting in a marked decrease of MRI T2-weighted signal intensity. Biodistribution studies demonstrated the efficient accumulation of SM-USPIO in the ch-hep-3 tumor in nude mice. The in vivo study in the ch-hep-3 tumor-bearing nude mice indicated that MRI using the SM-USPIO as contrast agent possessed good diagnostic ability, suggesting that SM-USPIO had the potential to be a promising targeted contrast agent for diagnosis of HCC. (C) 2008 Elsevier Inc. All rights reserved.