Dehydrocavidine attenuates D-galactose induced learning and memory impairment in rats
Dehydrocavidine attenuates D-galactose induced learning and memory impairment in rats
复制标题
脱氢卡维丁减轻 D-半乳糖诱导的大鼠学习和记忆障碍
DOI：
10.1016/j.neulet.2017.12.004
复制
发表时间：
2018-02-05
影响因子：
2.5
通讯作者：
Xiong, Nan-xiang
中科院分区：
文献类型：
--
作者：
Fu, Peng;Zhang, Qing;Xiong, Nan-xiang
目的：脱氢卡维啶（Dehydrocavidine，DEH）是岩黄连的主要成分，用于治疗肝病患者，但其对脑功能障碍的影响尚未见报道。本研究旨在探讨DEH对D-半乳糖（D-gala）所致大鼠学习记忆障碍的影响及其机制。采用D gala慢性腹腔注射和DEH灌胃建立大鼠学习记忆障碍模型，采用Morris水迷宫实验、Golgi染色和生化方法观察DEH对学习记忆障碍的影响，结果：腹腔注射D-gala可导致大鼠严重的学习记忆障碍，灌胃给予DEH可有效地减轻这种障碍。Golgi染色结果显示，添加DEH后，细胞棘密度恢复到5.7 ± 1.16个/10 μ m（p < 0.05）。补充DEH可逆转脂质过氧化反应，恢复酶活性，减轻氧化损伤（p < 0.05）。补充DEH可减少NO和PGE 2的产生以及iNOS和考克斯-2 mRNA的表达（p < 0.05）。结论：DEH可通过增强突触可塑性、减轻氧化损伤和限制神经炎症来减轻D-加拉诱导的大鼠学习记忆障碍。
Objective: Dehydrocavidine (DEH) is the major component of a plant corydalis saxicola Bunting, which is used to treat the patients with hepatic disorders, there is still no report about the effect of DEH on the brain disorder. In this study, the effect and mechanism of DEH on D-galactose (D-gala) induced learning and memory impairment in rats was investigated.Methods: A learning and memory impairment rat model was employed by chronic intraperitoneal injection of D gala and intragastric administration of DEH, then Morris water maze test was used to investigate the effect of DEH on learning and memory impairment, Golgi stain and biochemical methods, real time PCR were used to investigate underlying mechanism.Results: Intraperitoneal injection of D-gala could induced severe learning and memory impairment in rats, intragastric administration of DEH could effectively attenuates these deficits. Golgi staining showed DEH supplementary could restored the density of spines to 5.7 +/- 1.16 spines per 10 pm in the DEH + D-gala group (p < 0.05). DEH supplementary administration reversed the lipid peroxides and restored the enzymes activities to reduce oxidative damage (p < 0.05). DEH supplementary administration could reduced the production of NO and PGE2 and the mRNA expression of iNOS and COX-2 (p < 0.05).Conclusions: DEH could attenuates D-gala induced learning and memory impairment in rats by enhancing synaptic plasticity, reducing oxidative damage and limiting the neuroinflammation.