Involvement of a tobacco leucine-rich repeat-extensin in cell morphogenesis
Involvement of a tobacco leucine-rich repeat-extensin in cell morphogenesis
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DOI：
10.5511/plantbiotechnology.24.171
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发表时间：
2007-03
影响因子：
1.6
通讯作者：
H. Chida;K. Yazawa;S. Hasezawa;H. Iwai;S. Satoh
中科院分区：
文献类型：
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作者：
H. Chida;K. Yazawa;S. Hasezawa;H. Iwai;S. Satoh
利用单倍体白花烟草叶盘培养T-DNA转化产生的独特突变体nolac-K4(具有松散附着细胞的非器官发生愈伤组织)失去了形成不定芽的能力，并表现出细胞间附着减少。用T-DNA标记的基因命名为NpLRX1(LRR-EXTENSIN 1)，它是一个新的烟草基因，它编码一个含有嵌合的亮氨酸重复序列和延伸蛋白结构域的细胞壁蛋白。该基因与拟南芥LRX基因高度相似，系统发育分析将其归入营养LRX分支。NpLRX1在营养组织中普遍表达，在叶和根的维管束中表达强烈，在新生的侧根和根尖中表达。转化NpLRX1-RNAi的烟草叶盘出现不定芽异常，细胞形态发生紊乱，细胞间隙较大。NpLRX1-RNAi转化的NpLRX1-RNAi-2细胞形态不规则，皮质微管排列紊乱。这些结果表明，NpLRX1是一种新的烟草LRX基因，它在细胞形态发生中具有调节作用，而细胞形态发生是植物组织发育所必需的。
The unique mutant nolac-K4 (non-organogenic callus with loosely attached cells), which was generated by T- DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, has lost the ability to form adventitious shoots and also shows decreased intercellular attachment. The gene tagged with the T-DNA in this line, named NpLRX1 (LRR-EXTENSIN 1), is a novel tobacco gene that encodes a cell-wall protein containing chimeric leucine-rich repeat and extensin domains. The gene is highly similar to the Arabidopsis LRX genes, and phylogenetic analysis places it in the vegetative LRX clade. NpLRX1 is expressed ubiquitously in vegetative tissues, strongly in the leaf and root vascular bundles, and in emerging lateral roots and root tips. Tobacco leaf disks transformed with an NpLRX1-RNAi construct displayed aberrant adventitious buds and disorganized cell morphogenesis with large intercellular spaces. The shapes of NpLRX1- RNAi-transformed BY-2 cells were irregular, and the cells showed disorganized cortical microtubules. These results suggest that NpLRX1, the lack of which may be responsible for the nolac-K4 mutation, is a new tobacco LRX gene that has regulatory roles in cell morphogenesis that are essential for plant tissue development.