Characterisation of tectal ephrin-A2 expression during optic nerve regeneration in goldfish: implications for restoration of topography
Characterisation of tectal ephrin-A2 expression during optic nerve regeneration in goldfish: implications for restoration of topography
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DOI：
10.1016/j.expneurol.2004.02.006
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发表时间：
2004-06-01
影响因子：
5.3
通讯作者：
Beazley, L
中科院分区：
文献类型：
--
作者：
King, C;Lacey, R;Beazley, L
EphA受体和它们的配体肝配蛋白-As，表达为视网膜和顶盖梯度，是视网膜-顶盖地形图的发展[Neuron 25（2000）563]及其在金鱼视神经再生期间的恢复所需的[Mol. Cell. Neurosci. 25（2004）56]。我们先前已经报道，在再生期间，通过跨整个视网膜神经节细胞（RGC）群体的差异表达形成瞬时EphA 3/A5梯度[Neurosci. ABS. 33（2003）358.2; Exp. Neurol. 183（2003）593]。在retinorecipient顶盖层中，肝配蛋白-A2通常仅由细胞亚群表达，但在再生过程中，存在梯度增加，尾侧表达细胞多于吻侧[Exp. Neurol. 166（2000）196]。在这里，我们研究的特点，顶盖ephrin-A2的表达在再生过程中。我们报告说，肝配蛋白A2的表达水平是可比的所有肝配蛋白A2阳性细胞在正常动物和再生过程中。使用双标记免疫组织化学ephrin-A2和特定的细胞标记物（神经元，GAS星形胶质细胞，NN-1的小胶质细胞/内皮细胞和6D2的少突胶质细胞），我们证明ephrin-A2表达细胞，在正常动物中，是专门的神经元。此外，BrdU双标记显示肝配蛋白-A2在驻留细胞中表达，而不是在视神经再生期间产生的那些细胞中表达[Brain Res.854（2000）178，153（1978）345]。(C)2004年爱思唯尔公司All rights reserved.
EphA receptors and their ligands the ephrin-As, expressed as retinal and tectal gradients, are required for the development of retino-tectal topography [Neuron 25 (2000) 563] and its restoration during goldfish optic nerve regeneration [Mol. Cell. Neurosci. 25 (2004) 56]. We have reported previously that, during regeneration, a transient EphA3/A5 gradient is formed by differential expression across the entire retinal ganglion cell (RGC) population [Neurosci. Abs. 33 (2003) 358.2; Exp. Neurol. 183 (2003) 593]. In retinorecipient tectal layers, ephrin-A2 is normally expressed by only a sub-population of cells, but during regeneration, there is a graded increase with more expressing cells caudally than rostrally [Exp. Neurol. 166 (2000) 196]. Here, we examine the characteristics of tectal ephrin-A2 expression during regeneration. We report that the level of ephrin-A2 expression is comparable for all ephrin-A2-positive cells in normal animals and during regeneration. Using double-labelling immunohistochemistry for ephrin-A2 and specific cell markers (NeuN for neurons, GAS for astrocytes, NN-1 for microglia/endothelial cells and 6D2 for oligodendrocytes), we demonstrate that ephrin-A2-expressing cells, as in normal animals, are exclusively neuronal. Moreover, double labelling with BrdU showed that ephrin-A2 is expressed in resident cells and not those generated during optic nerve regeneration [Brain Res. 854 (2000) 178, 153 (1978) 345]. (C) 2004 Elsevier Inc. All rights reserved.