Culture media influenced laboratory outcomes but not neonatal birth weight in assisted reproductive technology
Culture media influenced laboratory outcomes but not neonatal birth weight in assisted reproductive technology
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DOI：
10.1007/s11596-015-1531-1
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Tailang Yin;Yi Zhang;Sai-jiao Li;Mengyan Zhao;Jinli Ding;Wang‐ming Xu;Jing Yang
中科院分区：
文献类型：
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作者：
Tailang Yin;Yi Zhang;Sai-jiao Li;Mengyan Zhao;Jinli Ding;Wang‐ming Xu;Jing Yang
研究了辅助生殖技术中使用的培养基类型是否对体外受精（IVF）/卵胞浆内单精子注射（ICSI）新生儿的实验室结局和出生体重产生影响。共纳入2010年1月1日至2012年12月31日期间接受IVF/ICSI并在新鲜胚胎移植后第3天分娩活单胎的673例患者。在此期间使用了三种类型的培养基：Quinn's Advantage（QA）、单步培养基（SSM）和连续单一培养基（CSC）。采用单因素方差分析和χ 2检验比较受精率（FR）、正常受精率（NFR）、卵裂率（CR）、正常卵裂率（NCR）、优质胚胎率（GQER）和新生儿出生体重。进行多元线性回归分析，以确定培养基对实验室结果和出生体重的影响。在IVF周期中，SSM培养液组GQER显著低于QA和CSC培养液组（QA组63.6%vs.69.0%vs.71.3%，P = 0.011）。在ICSI周期中，CSC培养基组的FR、NFR和CR均显著低于其他两组。3组新生儿出生体重差异无统计学意义（P = 0.759）。多元线性回归分析证实，培养基类型与FR、NFR、CR和GQER相关，而与新生儿出生体重无关。培养基类型对实验室结果有潜在影响，但对ART中单胎的出生体重没有影响。
Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns inin-vitrofertilization (IVF)/intracytoplasmic sperm injection (ICSI) was investigated. A total of 673 patients undergoing IVF/ICSI and giving birth to live singletons after fresh embryo transfer on day 3 from Jan. 1, 2010 to Dec. 31, 2012 were included. Three types of culture media were used during this period: Quinn’s Advantage (QA), Single Step Medium (SSM), and Continuous Single Culture medium (CSC). Fertilization rate (FR), normal fertilization rate (NFR), cleavage rate (CR), normal cleavage rate (NCR), good-quality embryo rate (GQER) and neonatal birth weight were compared using one-way ANOVA andχ2tests. Multiple linear regression analysis was performed to determine the impact of culture media on laboratory outcomes and birth weight. In IVF cycles, GQER was significantly decreased in SSM medium group as compared with QA or CSC media groups (63.6%vs. 69.0% in QA;vs. 71.3% in CSC,P=0.011). In ICSI cycles, FR, NFR and CR were significantly lower in CSC medium group than in other two media groups. No significant difference was observed in neonatal birthweight among the three groups (P=0.759). Multiple linear regression analyses confirmed that the type of culture medium was correlated with FR, NFR, CR and GQER, but not with neonatal birth weight. The type of culture media had potential influences on laboratory outcomes but did not exhibit an impact on the birth weight of singletons in ART.