Slipper snail tales: How Crepidula fornicata and Crepidula atrasolea became model molluscs.
Slipper snail tales: How Crepidula fornicata and Crepidula atrasolea became model molluscs.
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DOI：
10.1016/bs.ctdb.2021.12.013
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发表时间：
2022
影响因子：
--
通讯作者：
Henry, Jonathan Q
中科院分区：
文献类型：
--
作者：
Lyons, Deirdre C;Henry, Jonathan Q
尽管软体动物的数量和种类都很丰富，但只有少数软体动物获得了“模式研究生物”的地位。其中一个物种是拖鞋蜗牛Crepidula fornicata。它的胚胎在世纪末首次用于经典的谱系追踪研究，100多年后，它们被我们的实验室“重新发现”，并用于现代命运作图，基因扰动，体内成像，转录组学，以及在Spiralia/Lophotrochozoa中首次应用CRISPR/Cas9介导的基因组编辑。与此同时，其他实验室对包括Crepidula属在内的Calyptraeidae家族成员的分类学、生殖发育、生态学、生活史、发育模式、幼虫摄食行为和对环境的反应进行了广泛的研究。最近，我们开发了工具，资源和管理协议的另一个，直接发展的物种，Crepidula atrasolea。这个物种是软体动物中理想的“实验鼠”。这些物种一起将是有价值的探索细胞和分子机制的软体动物生物学和进化。
Despite the great abundance and diversity of molluscs, only a few have attained “model research organism” status. One of those species is the slipper snail Crepidula fornicata. Its embryos were first used for classical lineage tracing studies in the late 19th century, and over a 100 years later they were “re-discovered” by our labs and used for modern fate mapping, gene perturbation, in vivo imaging, transcriptomics, and the first application of CRISPR/Cas9-mediated genome editing among the Spiralia/Lophotrochozoa. Simultaneously, other labs made extensive examinations of taxonomy, phylogeny, ecology, life-history, mode of development, larval feeding behavior, and responses to the environment in members of the family Calyptraeidae, which includes the genus Crepidula. Recently, we developed tools, resources, and husbandry protocols for another, direct-developing species, Crepidula atrasolea. This species is an ideal “lab rat” among molluscs. Together these species will be valuable for probing the cellular and molecular mechanisms underlying molluscan biology and evolution.
影响因子：
4.6
作者：
Maboloc EA;Chan KYK
通讯作者：
Chan KYK