Optoacoustic mesoscopy analysis and quantitative estimation of specific imaging metrics in Fitzpatrick skin phototypes II to V
Optoacoustic mesoscopy analysis and quantitative estimation of specific imaging metrics in Fitzpatrick skin phototypes II to V
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DOI：
10.1002/jbio.201800442
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发表时间：
2019-09-01
影响因子：
2.8
通讯作者：
Olivo, Malini
中科院分区：
文献类型：
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作者：
Li, Xiuting;Dinish, U. S.;Olivo, Malini
光栅扫描光声介观镜 (RSOM) 是一种新型光声成像方式，可提供皮下 1 至 2 毫米的非侵入性、无标记、高分辨率（~7 μm 轴向，~30 μm 横向）成像，为皮肤病理生理学提供新颖的定量见解。由于 RSOM 图像对比度机制基于光吸收，因此预计皮肤中存在的黑色素量将影响 RSOM 图像。然而，迄今为止，肤色对 RSOM 性能的影响尚未得到解决。在此，我们展示了 RSOM 对具有 II 至 V 皮肤类型的菲茨帕特里克 (FP) 皮肤类型的人类受试者进行体内皮肤成像的效率。从受试者前臂掌侧获取的 RSOM 图像用于导出 RSOM 研究中使用的指标，例如总血量、血管直径和黑色素信号强度。我们的研究表明，对于不同 FP 皮肤类型的受试者，从 RSOM 图像得出的黑色素信号强度与从临床色度计获得的黑色素信号强度表现出极好的相关性。我们可以成功地估计真皮不同深度的血管直径。此外，我们的研究表明，由于黑色素对光的强烈吸收，真皮中的信噪比较低，因此需要对 FP 皮肤类型较高的受试者计算的总血量进行补偿。这项研究揭示了如何使用 RSOM 研究具有不同皮肤表型的人群的各种皮肤状况。
Raster Scanning Optoacoustic Mesoscopy (RSOM) is a novel optoacoustic imaging modality that offers non-invasive, label-free, high resolution (~7 mu m axial, ~30 mu m lateral) imaging up to 1 to 2 mm below the skin, providing novel quantitative insights into skin pathophysiology. As the RSOM image contrast mechanism is based on light absorption, it is expected that the amount of melanin present in the skin will affect RSOM images. However, the effect of skin tone in the performance of RSOM has not been addressed so far. Herein, we present the efficiency of RSOM for in vivo skin imaging of human subjects with Fitzpatrick (FP) skin types between II to V. RSOM images acquired from the volar forearms of the subjects were used to derive metrics used in RSOM studies, such as total blood volume, vessel diameter and melanin signal intensity. Our study shows that the melanin signal intensity derived from the RSOM images exhibited an excellent correlation with that obtained from a clinical colorimeter for the subjects of varying FP skin types. We could successfully estimate the vessel diameter at different depths of the dermis. Furthermore, our study shows that there is a need to compensate for total blood volume calculated for subjects with higher FP skin types due to the lower signal-to-noise ratio in dermis, owing to strong absorption of light by melanin. This study sheds light into how RSOM can be used for studying various skin conditions in populations with different skin phenotypes.