Two types of core/shell fibers based on carboxymethyl chitosan and Sodium carboxymethyl cellulose with self-assembled liposome for buccal delivery of carvedilol across TR146 cell culture and porcine buccal mucosa
Two types of core/shell fibers based on carboxymethyl chitosan and Sodium carboxymethyl cellulose with self-assembled liposome for buccal delivery of carvedilol across TR146 cell culture and porcine buccal mucosa
复制标题
DOI：
10.1016/j.ijbiomac.2019.01.143
复制
发表时间：
2019-05-01
影响因子：
8.2
通讯作者：
Pan, Weisan
中科院分区：
文献类型：
--
作者：
Chen, Jianting;Duan, Hongliang;Pan, Weisan
分别基于生物粘附聚合物羧甲基壳聚糖（CMCS）和羧甲基纤维素钠（CMC-Na）制备了两种新型可自组装脂质体的核壳纤维。壳层分别为CMCS/PVA和CMC-Na/PVA，芯层为PVP、磷脂(PC)和卡维地洛(Car)的混合物。通过SEM和CLSM检查直径分布和核/壳结构。 FFIR 和 XRD 也用于表征光纤。通过 TEM 观察自组装脂质体，并测定药物包封率等其他参数。通过体外粘附力来评价纤维的生物粘附性能。溶出度测试表明，Car在2小时内几乎完全释放，并呈线性释放。对猪 TR146 细胞培养物和颊粘膜的渗透研究表明，自组装脂质体和生物粘附聚合物均促进药物渗透。 MTT测试显示TR146细胞与浓度为10 mg/mL的纤维提取液孵育后是安全的。总之，这种基于使用水溶性生物粘附聚合物的自组装脂质体和核/壳纤维的设计对于汽车口腔吸收是理想的。 (C) 2019 年由 Elsevier B.V. 出版
Two novel core-shell fibers which could self-assemble liposome were fabricated based on bioadhesive polymer carboxymethyl chitosan (CMCS) and Sodium carboxymethyl cellulose (CMC-Na), separately. The shell layers were CMCS/PVA and CMC-Na/PVA, respectively, and the core layer was mixture of PVP, Phospholipids (PC) and Carvedilol (Car). The diameter distribution and core/shell structure were examined by SEM and CLSM. FFIR and XRD were also used to characterize the fiber. Self-assembled liposome was observed by TEM, and other parameters like drug encapsulation efficiency was also determined. In vitro adhesive force was conducted to evaluate bioadhesive property of fibers. Dissolution test demonstrated Car was almost completely released within 2 h and presented linear release. The permeation studies across porcine TR146 cell culture and buccal mucosa were carried out, indicating self-assembled liposome and bioadhesive polymer both promoted drug penetration. MTT test showed TR146 cells were safe after incubation with fibers' extraction medium under the concentration of 10 mg/mL. In summary, this design based on self-assembled liposome and core/shell fiber using water-soluble bioadhesive polymer was desirable for Car buccal absorption. (C) 2019 Published by Elsevier B.V.