A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation
A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation
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DOI：
10.1074/jbc.m212212200
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发表时间：
2003-05-09
影响因子：
4.8
通讯作者：
Yuasa, Y
中科院分区：
文献类型：
--
作者：
Miyake, S;Yanagisawa, Y;Yuasa, Y
EID-1（（E）在条1A下-样（i）在条1A下-样（d）在条1A下-样（1）在条1A下-样（i）在条1A下-样（1）在条1A下-样（d）在条1A下-样（1）在条1A下-样（i）在条1A下-样（1）在条1A下-样（d）在条1A下-样（1）在条1A下-样（1）在条1A下-样抑制剂）通过阻断p300的组蛋白乙酰转移酶活性来抑制分化。在这里，我们报告了一种新的分化抑制剂，表现出与EID-1的同源性，称为EID-2（（E）在bar ID-1下-样（i）在bar抑制剂下（d）在bar分化下-（2）在bar下）。EID-2抑制MyoD依赖性转录和肌肉分化。与EID-1不同，EID-2不阻断p300活性。有趣的是，EID-2与I类组蛋白脱乙酰酶（HDAC）相关。EID-2的N-末端部分是与HDAC结合所必需的。该区域还参与转录抑制和核定位，表明HDAC参与EID-2功能的重要性。这些结果表明一个新的分化抑制剂家族，尽管EID-2和EID-1之间的生化机制存在一些差异。
An EID-1 ((E) under bar 1A-like (i) under bar nhibitor of (d) under bar ifferentiation-(1) under bar) inhibits differentiation by blocking the histone acetyltransferase activity of p300. Here we report a novel inhibitor of differentiation exhibiting homology to EID-1, termed EID-2 ((E) under bar ID-1- like (i) under bar nhibitor of (d) under bar ifferentiation-(2) under bar). EID-2 inhibited MyoD-dependent transcription and muscle differentiation. Unlike EID-1, EID-2 did not block p300 activity. Interestingly, EID-2 associated with class I histone deacetylases (HDACs). The N-terminal portion of EID-2 was required for the binding to HDACs. This region was also involved in the transcriptional repression and nuclear localization, suggesting the importance of the involvement of HDACs in the EID-2 function. These results indicate a new family of differentiation inhibitors, although there are several differences in the biochemical mechanisms between EID-2 and EID-1.