Production of bacteriocin-like inhibitory compounds by human fecal Bifidobacterium strains
Production of bacteriocin-like inhibitory compounds by human fecal Bifidobacterium strains
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DOI：
10.4315/0362-028x-68.5.1034
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发表时间：
2005-05-01
影响因子：
2
通讯作者：
Sanz, Y
中科院分区：
文献类型：
--
作者：
Collado, MC;Hernández, M;Sanz, Y
从人粪便中分离出耐酸和耐胆汁的双歧杆菌菌株，并通过属特异性PCR和随机扩增多态性DNA PCR进行鉴定。通过测定其中和培养上清液的抑制作用，筛选了24种不同的菌株，用于可能产生蛋白质抗菌化合物。根据双歧杆菌的广谱抑菌谱，筛选出6株双歧杆菌菌株（BIR-0304、BIR-0307、BIR-0312、BIR-0324、BIR-0326和BIR-0349）。这些菌株对与食品安全和人类健康相关的革兰氏阳性菌、革兰氏阴性菌和酵母菌具有抗菌活性。六种选择的双歧杆菌菌株的拮抗作用与细菌素样化合物有关，所述细菌素样化合物在pH值3至10之间具有活性，在100 ℃下稳定10分钟，对α-淀粉酶和脂肪酶A具有抗性，但对蛋白酶（胰蛋白酶、蛋白酶K、蛋白酶A、胃蛋白酶和组织蛋白酶13）敏感。由双歧杆菌BIR-0312和BIR-0324产生的抗微生物化合物的分子量在10至30 kDa的范围内，而由双歧杆菌BIR-0304、BIR-0307、BIR-0326和BIR-0349产生的化合物的分子量小于10 kDa。所有双歧杆菌菌株在对数生长期后期和吐温80的存在下产生最大的抗菌活性。这些结果证实了类细菌素抑制化合物的合成是双歧杆菌菌株体外抑制病原菌和腐败菌的关键因素。
Acid- and bile-resistant Bifidobacterium strains were isolated from human feces and identified by genus-specific PCR and randomly amplified polymorphic DNA PCR. Twenty-four different strains were screened for possible production of proteinaccous antimicrobial compounds by assaying the inhibitory effects of their neutralized culture supernatants. Six Bifidobacterium strains (BIR-0304, BIR-0307, BIR-0312, BIR-0324, BIR-0326, and BIR-0349) were selected on the basis of their broad inhibitory spectra. These strains were active against gram-positive and gram-negative bacteria and yeasts relevant to food safety and human health. The antagonistic effects of the six selected Bifidobacterium strains were related to bacteriocin-like compounds, which were active at pH values between 3 and 10, stable at 100 degrees C for 10 min, resistant to a-amylase and lipase A, but sensitive to protemases (trypsin, proteinase K, protease A, pepsin, and cathepsin 13). The molecular masses of the antimicrobial compounds produced by Bifidobacterium BIR-0312 and BIR-0324 were in the range of 10 to 30 kDa, and those of the compounds produced by Bifidobacterium BIR-0304, BIR-0307, BIR-0326, and BIR-0349 were less than 10 kDa. All Bifidobacterium strains produced maximum antimicrobial activities in the late logarithmic phase of growth and in the presence of Tween 80. These results confirm that the synthesis of bacteriocin-like inhibitory compounds is a key factor in the in vitro inhibition of pathogen and spoilage bacteria by Bifidobacterium strains.