Microbiota of field-collected Ixodes scapularis and Dermacentor variabilis from eastern and southern Ontario, Canada
Microbiota of field-collected Ixodes scapularis and Dermacentor variabilis from eastern and southern Ontario, Canada
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DOI：
10.1016/j.ttbdis.2017.09.009
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发表时间：
2018-02-01
影响因子：
3.2
通讯作者：
Jardine, Claire M.
中科院分区：
文献类型：
--
作者：
Clow, Katie M.;Weese, J. Scott;Jardine, Claire M.
由于更广泛的微生物群落在病原体携带和传播中可能发挥的潜在作用，硬蜱的微生物群一直是人们越来越感兴趣的领域。在过去的二十年中，加拿大安大略的蜱传播疾病的风险发生了快速变化，主要是由于肩胛硬蜱的范围扩大。另一种咬人的动物Dermacentor variabilis是该省的长期居民，但目前病原体传播的风险很小。为了研究这两个物种的微生物群，我们收集了成虫和若虫I。scapularis和D.通过蜱拖曳从该省东部和南部地区采集变异蜱，并通过Illumina MiSeq对19个样品（由45只蜱组成）进行下一代测序。共检测到1400469个序列（中位数69118/样品;范围23350-155227）。未分类的梭菌目和瘤胃球菌科是I. scapularis和D.变量任何门，纲，目，科或属的相对丰度没有显着差异，检测位置（东与南），性别，生活阶段或蜱种。根据unifrac和AMOVA分析，社区成员或结构没有差异。根据辛普森指数和香农均匀度指数，雌性和雄性蜱的微生物多样性较低。我们的研究结果与以前在其他地理区域对这些蜱虫物种进行的研究不同，并强调了地理和相关生态因素在塑造蜱虫微生物群方面可能发挥的潜在作用。
The microbiota of hard ticks has been an area of growing interest due to the potential role that the broader microbial community may play in pathogen carriage and transmission. In the last two decades, Ontario, Canada has experienced rapid changes in the risk of tick-borne disease, primarily due to the range expansion of Ixodes scapularis. Another human-biter, Dermacentor variabilis, is a longstanding resident of the province, but currently poses minimal risk of pathogen transmission. To examine the microbiota of these two species, we collected adult and nymphal I. scapularis and D. variabilis from the eastern and southern regions of the province via tick dragging, and conducted next generation sequencing of 19 samples (composed of 45 ticks) via Illumina MiSeq. A total of 1400469 sequences were detected (median 69118/sample; range 23350-155227). The most abundant families of bacteria were unclassified Clostridiales and Ruminococcaceae for both I. scapularis and D. variabilis. No significant differences in the relative abundances of any phylum, class, order, family or genus were detected between locations (east vs south), sex, life stage or tick species. There were no differences in community membership or structure based on unifrac and AMOVA analyses. Female and male ticks had lower microbial diversity when compared to nymphs, based on the Simpson's index and Shannon evenness index. The findings of our study differ from previous studies of these tick species conducted in other geographic areas, and highlight the potential role geography and related ecological factors may have in shaping the tick microbiota.