Cocaine-Induced Abnormal Cerebral Hemodynamic Responses to Forepaw Stimulation Assessed by Integrated Multi-Wavelength Spectroimaging and Laser Speckle Contrast Imaging
Cocaine-Induced Abnormal Cerebral Hemodynamic Responses to Forepaw Stimulation Assessed by Integrated Multi-Wavelength Spectroimaging and Laser Speckle Contrast Imaging
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DOI：
10.1109/jstqe.2015.2503319
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发表时间：
2016-07-01
影响因子：
4.9
通讯作者：
Du, Congwu
中科院分区：
文献类型：
--
作者：
Chen, Wei;Park, Kicheon;Du, Congwu
药物中毒期间脑血流动力学变化对功能激活反应的同步成像为评估可卡因诱导的神经血管功能障碍提供了一种有价值的策略。然而，这需要具有足够的时空分辨率和足够的信噪比（SNR）的工具。虽然已经开发了几种技术来解决功能性大脑激活期间的这一需求，但它们的时空分辨率已经受到损害，以保持信噪比。在本研究中，我们将时空激光散斑对比分析和图像相关技术相结合，将多波长光谱成像和激光散斑对比成像（MW-LSCI）相结合。实验结果表明，优化的时空分辨率和增强的信噪比能够同时测量可卡因给药期间的多种血流动力学反应（即Delta HbO(2)、Delta HbR、Delta HbT和Delta CBF）。具体而言，通过测量可卡因给药后不同时间前爪电刺激的激活反应，可以了解可卡因诱导的功能性脑血流动力学变化。该系统具有更高的时空分辨率和信噪比，能够区分可卡因对脑血管系统和组织代谢影响的异质性，显示了MW-LSCI在各种脑功能和药理研究中的独特能力。
Simultaneous imaging of cerebral hemodynamic changes in response to functional activation during drug intoxication provides a valuable strategy to assess cocaine-induced neurovascular dysfunction. However, this requires tools with sufficient spatiotemporal resolution and an adequate signal to noise ratio (SNR). Though several technologies have been developed to address this demand during functional brain activation, their spatiotemporal resolution has been compromised to preserve SNR. In this study, we combine spatiotemporal-domain laser speckle contrast analysis and image correlation techniques to integrate the multi-wavelength spectroimaging and laser speckle contrast imaging (MW-LSCI). Experimental results show that optimized spatiotemporal resolution with enhanced SNR were achieved that enabled simultaneous measurement of multiple hemodynamic responses (i.e., Delta HbO(2), Delta HbR, Delta HbT, and Delta CBF) during cocaine administration. Specifically, cocaine-induced functional cerebral hemodynamic changes were accessed by measuring the activation responses to forepaw electrical stimulation at different times after cocaine administration. With an improved spatiotemporal resolution and SNR, the system was able to differentiate the heterogeneity of cocaine's effects on the cerebral vasculature and on tissue metabolism, demonstrating the unique capability of MW-LSCI for various brain functional and pharmacological studies.