Correlation between concentrations of 8-oxo-7,8-dihydro-2′-deoxyguanosine in urine, plasma and saliva measured by on-line solid-phase extraction LC-MS/MS
Correlation between concentrations of 8-oxo-7,8-dihydro-2′-deoxyguanosine in urine, plasma and saliva measured by on-line solid-phase extraction LC-MS/MS
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DOI：
10.1016/j.cca.2010.04.029
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发表时间：
2010-09-06
影响因子：
5
通讯作者：
Chao, Mu-Rong
中科院分区：
文献类型：
--
作者：
Hu, Chiung-Wen;Huang, Yu-Jie;Chao, Mu-Rong
背景：8-氧-7,8-二氢-2'-脱氧鸟苷（8-oxodGuo）是氧化应激最常用的生物标志物。基于色谱法测定尿液中8-氧化郭的方法已经建立；然而，血浆和唾液中8-氧化过的测定一直存在问题。方法：采用在线固相萃取（SPE）液相色谱-质谱联用技术（LC-MS/MS）对血浆和唾液中的8-oxodGuo进行定量分析。同时采集50名健康成人的尿液、血浆和唾液标本，测定8-氧过含量。结果：8-oxodGuo在血浆和唾液中的平均基线水平分别为21.7 +/- 9.2和5.1 +/- 2.6 pg/ml，远低于尿中（6.2 +/- 4.8 ng/ml）。然而，本研究在血浆和唾液中获得的8-oxodGuo水平比文献中商用ELISA试剂盒报道的水平低了数百倍。此外，血浆和唾液中的8-oxodGuo水平与尿液中的8-oxodGuo水平显著相关（Spearman相关系数，血浆r = 033, P = 0.02，唾液r = 0.56,P = 0.0015）。血浆中8-OxodGuo与唾液中8-OxodGuo也有相关性（r = 0.52， P= 0.0041）。结论：观察到血浆、唾液和尿液之间存在显著相关性，从而有可能使用尿液以外的其他体液来评估全身氧化应激。(C) 2010 Elsevier B.V.版权所有
Background: 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is the most frequently measured biomarker of oxidative stress. Chromatographic-based methods for 8-oxodGuo in urine are well established; however, the 8-oxodGuo measurement in plasma and saliva has been problematic.Methods: We firstly and successfully applied an on-line solid-phase extraction (SPE) LC-MS/MS following manual SPE pretreatment to quantify the 8-oxodGuo both in plasma and saliva. Urine, plasma and saliva specimens were simultaneously collected from 50 healthy adults and measured for 8-oxodGuo.Results: Mean baseline levels of 8-oxodGuo in plasma and saliva were 21.7 +/- 9.2 and 5.1 +/- 2.6 pg/ml, respectively, being far lower than that in urine (6.2 +/- 4.8 ng/ml). The 8-oxodGuo levels obtained in this study for plasma and saliva were, however, up to several hundred times lower than those reported by commercial ELISA kit in the literature. Furthermore, the 8-oxodGuo levels in plasma and saliva were significantly correlated with the 8-oxodGuo levels in urine (Spearman correlation coefficients, r = 033, P = 0.02 for plasma and r = 0.56,P = 0.0015 for saliva). 8-OxodGuo in plasma was also correlated with the 8-oxodGuo in saliva (r = 0.52, P= 0.0041).Conclusions: Significantly correlations were observed between plasma, saliva and urine, giving the possibility of using other body fluids in addition to urine for assessing whole body oxidative stress. (C) 2010 Elsevier B.V. All rights reserved.