Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
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DOI：
10.1126/sciadv.aat0496
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发表时间：
2018-05-01
期刊：
影响因子：
13.6
通讯作者：
Sikulu-Lord, Maggy T.
中科院分区：
文献类型：
--
作者：
Fernandes, Jill N.;dos Santos, Lilha M. B.;Sikulu-Lord, Maggy T.
寨卡病毒（ZIKV）等虫媒病毒在全球的加速传播，凸显了对蚊子进行更积极主动监测的必要性。然而，虫媒病毒爆发期间的一个主要挑战是缺乏快速和负担得起的蚊子病原体检测方法。我们首次表明，近红外光谱（NIRS）是一种快速，无试剂，具有成本效益的工具，可用于非侵入性检测ZIKV在完整的埃及伊蚊蚊子的头部和胸部与94.2%至99.3%的预测精度相对于定量逆转录聚合酶链反应（RT-qPCR）。近红外光谱技术只需将一束光照射在蚊子身上，就可以收集诊断光谱。在这项研究中，我们估计NIRS比RT-qPCR快18倍，便宜110倍。我们预计，近红外光谱将扩大后，确定潜在的虫媒病毒的热点，以指导空间优先级的载体控制。
The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.