Synergism of α-Linolenic Acid, Conjugated Linoleic Acid and Calcium in Decreasing Adipocyte and Increasing Osteoblast Cell Growth
Synergism of α-Linolenic Acid, Conjugated Linoleic Acid and Calcium in Decreasing Adipocyte and Increasing Osteoblast Cell Growth
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DOI：
10.1007/s11745-013-3803-5
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发表时间：
2013-08-01
期刊：
影响因子：
1.9
通讯作者：
Ilich, Jasminka Z.
中科院分区：
文献类型：
--
作者：
Kim, Youjin;Kelly, Owen J.;Ilich, Jasminka Z.
全脂牛奶和乳制品（尽管与低脂或脱脂产品相比提供更多能量）是α-亚麻酸（ALA），共轭亚油酸（CLA）和钙的良好来源，这可能有利于调节骨骼和脂肪组织代谢。我们研究了ALA，CLA和钙（水平类似于全脂牛奶/乳制品）在调节骨和脂肪细胞生长中的单独和/或协同作用。用以下物质处理ST 2基质、MC 3 T3-L1脂肪细胞样和MC 3 T3-E1成骨细胞样细胞：（a）亚油酸（LNA）：ALA比例= 1-5：1;（B）单独/组合的80- 90%c9，t11（9，11）和5- 10%t10，c12（10，12）CLA异构体;（c）0.5-3.0 mM钙;（d）（a）、（B）、（c）的组合;及（e）控制。测量局部介质，包括类花生酸和生长因子。(a)在4：1的LNA：ALA比例下发现最佳效果，其中胰岛素样生长因子-1（IGF-1）和IGF结合蛋白-3（IGFBP-3）的产生在MC 3 T3-L1细胞中最低。(b)所有CLA异构体共混物降低MC 3 T3-L1并增加MC 3 T3-E1细胞分化。(c)1.5-2.5 mM钙增加ST 2和MC 3 T3-E1，并降低MC 3 T3-L1细胞增殖。(d)4：1 LNA：ALA + 90：10%CLA + 2.0 mM钙的组合降低了MC 3 T3-L1并增加了MC 3 T3-E1细胞分化。总体而言，增强成骨细胞生成和抑制脂肪生成的最佳LNA：ALA比例为4：1。90：10%CLA +2.0mM钙增强了这种作用，表明这些饮食因子在细胞培养物中促进成骨细胞和抑制脂肪细胞分化方面可能具有协同作用。
Whole fat milk and dairy products (although providing more energy compared to low- or non-fat products), are good sources of alpha-linolenic acid (ALA), conjugated linoleic acid (CLA) and calcium, which may be favorable in modulating bone and adipose tissue metabolism. We examined individual and/or synergistic effects of ALA, CLA and calcium (at levels similar to those in whole milk/dairy products) in regulating bone and adipose cell growth. ST2 stromal, MC3T3-L1 adipocyte-like and MC3T3-E1 osteoblast-like cells were treated with: (a) linoleic acid (LNA):ALA ratios = 1-5:1; (b) individual/combined 80-90 % c9, t11 (9,11) and 5-10 % t10, c12 (10,12) CLA isomers; (c) 0.5-3.0 mM calcium; (d) combinations of (a), (b), (c); and (e) control. Local mediators, including eicosanoids and growth factors, were measured. (a) The optimal effect was found at the 4:1 LNA:ALA ratio where insulin-like growth factor-1 (IGF-1) and IGF binding protein-3 (IGFBP-3) production was the lowest in MC3T3-L1 cells. (b) All CLA isomer blends decreased MC3T3-L1 and increased MC3T3-E1 cell differentiation. (c) 1.5-2.5 mM calcium increased ST2 and MC3T3-E1, and decreased MC3T3-L1 cell proliferation. (d) Combination of 4:1 LNA:ALA + 90:10 % CLA + 2.0 mM calcium lowered MC3T3-L1 and increased MC3T3-E1 cell differentiation. Overall, the optimal LNA:ALA ratio to enhance osteoblastogenesis and inhibit adipogenesis was 4:1. This effect was enhanced by 90:10 % CLA + 2.0 mM calcium, indicating possible synergism of these dietary factors in promoting osteoblast and inhibiting adipocyte differentiation in cell cultures.