Current status of viroid taxonomy
Current status of viroid taxonomy
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DOI：
10.1007/s00705-014-2200-6
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发表时间：
2014-12-01
影响因子：
2.7
通讯作者：
Owens, R. A.
中科院分区：
文献类型：
--
作者：
Di Serio, F.;Flores, R.;Owens, R. A.
类病毒是迄今为止已知的最小的自主感染性核酸。类病毒具有约250-400 nt的小型环状RNA基因组，其显然不编码任何蛋白质，类病毒在宿主植物中系统地复制和移动。自从近45年前发现第一个类病毒以来，许多不同的类病毒已被分离、表征，并经常被鉴定为植物病害的病原体。第一个类病毒分类方案是在20世纪90年代初提出的，几年后被国际病毒分类委员会（ICTV）采纳。在这里，目前的类病毒分类方案和类病毒种划分的标准进行了讨论，突出了目前正在审议的ICTV类病毒研究组的主要分类问题。正确的类病毒序列变异的分类注释的影响也得到解决，考虑到下一代测序和生物信息学已知的和以前未识别的类病毒的应用越来越多。
Viroids are the smallest autonomous infectious nucleic acids known so far. With a small circular RNA genome of about 250-400 nt, which apparently does not code for any protein, viroids replicate and move systemically in host plants. Since the discovery of the first viroid almost forty-five years ago, many different viroids have been isolated, characterized and, frequently, identified as the causal agents of plant diseases. The first viroid classification scheme was proposed in the early 1990s and adopted by the International Committee on Taxonomy of Viruses (ICTV) a few years later. Here, the current viroid taxonomy scheme and the criteria for viroid species demarcation are discussed, highlighting the main taxonomic questions currently under consideration by the ICTV Viroid Study Group. The impact of correct taxonomic annotation of viroid sequence variants is also addressed, taking into consideration the increasing application of next-generation sequencing and bioinformatics for known and previously unrecognized viroids.