Non-disjunction and Chromosome Loss in Gamma-Irradiated Human Lymphocytes: A Fluorescence In Situ Hybridization Analysis Using Centromere-Specific Probes
Non-disjunction and Chromosome Loss in Gamma-Irradiated Human Lymphocytes: A Fluorescence In Situ Hybridization Analysis Using Centromere-Specific Probes
复制标题
伽马射线照射的人淋巴细胞中的不分离和染色体丢失：使用着丝粒特异性探针的荧光原位杂交分析
作者：
I. Ponsa;Joan Francesc Barquinero;R. Miró;J. Egozcue;A. Genescà
摘要Ponsa，I.，Barquinero，J.F.，米罗河Egozcue，J.和Genescà，A.γ射线照射后人淋巴细胞的染色体不分离和丢失：着丝粒特异性探针荧光原位杂交分析。用4、7和18号染色体着丝粒特异性DNA探针同时分析了γ射线诱发的双核人淋巴细胞染色体丢失、不分离、标记区断裂和核质桥。使用的剂量为0、1、2和4戈伊，并且每剂量对大约1000个细胞进行评分。微核率呈线性二次型增加。对于染色体丢失，在2和4戈伊剂量下观察到显著增加，而对于非分离，在1戈伊剂量下观察到显著增加;因此，非分离使我们能够在比染色体丢失更低的剂量下检测辐射的影响。使用着丝粒特异性探针可以区分电离辐射的致染色体断裂和非整倍体效应。不考虑片段化信号的染色体丢失分析确保了非整倍体效应的检测，这是使用泛着丝粒探针不可能的。标记区域内的染色体断裂频率在细胞核中高于微核中，这表明由于培养物中存在细胞松弛素B，细胞核对染色体物质的吞噬增加。在辐照样品中观察到连接主核的染色质丝，即所谓的核质桥，是染色体重排产生后期桥的表现。
Abstract Ponsa, I., Barquinero, J. F., Miró, R., Egozcue, J. and Genescà, A. Non-disjunction and Chromosome Loss in Gamma-Irradiated Human Lymphocytes: A Fluorescence In Situ Hybridization Analysis Using Centromere-Specific Probes. Centromere-specific DNA probes for chromosomes 4, 7 and 18 were used to simultaneously analyze chromosome loss, non-disjunction, breaks within the labeled region, and nucleoplasmic bridges induced by γ rays in binucleated human lymphocytes. The doses used were 0, 1, 2 and 4 Gy, and approximately 1000 cells were scored per dose. Micronucleus frequency increased in a linear-quadratic fashion. For chromosome loss, significant increases were observed at 2 and 4 Gy, whereas for non-disjunction significant increases were observed at 1 Gy; thus non-disjunction allowed us to detect the effects of radiation at a lower dose than chromosome loss. The use of centromere-specific probes allowed discrimination between the clastogenic and aneugenic effects of ionizing radiation. The analysis of chromosome loss, not taking fragmented signals into account, ensures the detection of an aneugenic effect, which was not possible using pancentromeric probes. The frequency of chromosome breakage within the labeled regions was higher in nuclei than in micronuclei, suggesting an increase in the engulfment of chromosomal material by nuclei as a consequence of the presence of cytochalasin B in the cultures. Chromatin filaments connecting main nuclei, the so-called nucleoplasmic bridges, were observed in irradiated samples, and are a manifestation of rearranged chromosomes producing anaphase bridges.
影响因子：
3.4
作者：
MILLER, BM;WERNER, T;NUSSE, M
通讯作者：
NUSSE, M