Human iPS Cell-Derived Cardiac Tissue Sheets: a Platform for Cardiac Regeneration.
Human iPS Cell-Derived Cardiac Tissue Sheets: a Platform for Cardiac Regeneration.
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DOI：
10.1007/s11936-016-0489-z
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发表时间：
2016-11
影响因子：
--
通讯作者：
Yamashita, Jun K
中科院分区：
文献类型：
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作者：
Masumoto, Hidetoshi;Yamashita, Jun K
干细胞治疗是一种有前途的治疗选择，严重的心脏疾病是抵抗传统疗法。为了克服大多数临床研究对干细胞注射到受损心脏的不满意的结果，我们正在开发使用多能干细胞衍生的心肌细胞和血管细胞的生物工程心脏组织移植物。我们已经在大鼠心肌梗死模型中验证了小鼠胚胎干细胞衍生的和人诱导多能干细胞衍生的心脏组织片（CTS）的功能益处。我们进一步显示了增强的功能恢复和移植效率，导致在移植层之间具有明胶水凝胶微球的厚的多层CTS时新生心肌。我们预计，多能干细胞生物学和组织工程学的结合将有助于未来严重心脏病的干细胞疗法。
Stem cell therapy is a promising therapeutic option for severe cardiac diseases that are resistant to conventional therapies. To overcome the unsatisfactory results of most clinical researches on stem cell injections to an injured heart, we are developing bioengineered cardiac tissue grafts using pluripotent stem cell-derived cardiomyocytes and vascular cells. We have validated the functional benefits of mouse embryonic stem cell-derived and human induced pluripotent stem cell-derived cardiac tissue sheets (CTSs) in a rat myocardial infarction model. We further showed enhanced functional recovery and engraftment efficiency leading to de novo myocardium upon transplanting thick multi-layered CTSs that had gelatin hydrogel microspheres between the layers. We anticipate that the combination of pluripotent stem cell biology and tissue engineering will contribute to future stem cell therapies for severe heart diseases.