A SOLID-PHASE IMMUNOENZYMATIC TECHNIQUE FOR THE ENUMERATION OF SPECIFIC ANTIBODY-SECRETING CELLS
A SOLID-PHASE IMMUNOENZYMATIC TECHNIQUE FOR THE ENUMERATION OF SPECIFIC ANTIBODY-SECRETING CELLS
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DOI：
10.1016/0022-1759(83)90091-1
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
HOLT, PG
中科院分区：
文献类型：
--
作者：
SEDGWICK, JD;HOLT, PG
基于 ELISA [酶联免疫吸附测定] 的完善原理，描述了一种灵敏的免疫测定法，用于检测独特型和同种型特异性[大鼠]抗体分泌细胞 (ASC)。含有 ASC 的单细胞悬浮液在固相上孵育，特定抗原 [牛血清白蛋白、卵清蛋白、卵类粘蛋白] 已化学结合。抗体在 ASC 的直接微环境中附着在后者上，产生结合抗体的局部区域，随后在 ELISA 中形成可视点。该测定系统具有至少相当于溶血斑测定的灵敏度和特异性。
A sensitive immunoassay is described for the detection of idiotype- and isotype-specific [rat] antibody-secreting cells (ASC), based upon the well established principles of ELISA [enzyme-linked immunosorbent assay]. Single cell suspensions containing ASC are incubated on solid phase to which specific antigen [bovine serum albumin, ovalbumin, ovomucoid] has been chemically conjugated. Antibody attaches to the latter within the immediate microenvironment of the ASC, producing localized zones of bound antibody which are subsequently developed as visual spots in the ELISA. This assay system has sensitivity and specificity at least equivalent to hemolytic plaque assays.