The effects of coronavirus on human nasal ciliated respiratory epithelium
The effects of coronavirus on human nasal ciliated respiratory epithelium
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DOI：
10.1183/09031936.01.00093001
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发表时间：
2001-12-01
影响因子：
24.3
通讯作者：
O'Callaghan, C
中科院分区：
文献类型：
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作者：
Chilvers, MA;McKean, M;O'Callaghan, C
人类冠状病毒（HCoV）占普通感冒的15-30%，但只有一例报道描述了无症状的冠状病毒感染对人类呼吸道上皮的影响。作者研究了HCoV感染对鼻内接种HCoV 229 E的健康志愿者纤毛结构和功能的影响。另外四名志愿者用紫外线灭活病毒进行假感染。接种前即刻（第0天）和接种后3天（第3天），通过刷拭下鼻甲获得纤毛上皮。使用数字高速视频摄影测定纤毛搏动频率并分析搏动模式以获得运动障碍的证据（0=正常，3=严重运动障碍）。透射电镜观察纤毛超微结构。所有接种HCoV的受试者，包括未出现上呼吸道感染症状的三名受试者，在第3天呼吸道上皮细胞破裂。虽然第0天（11.3 Hz（95% CI：8.6-14.0））和第3天（9.4 Hz（95% CI：7.2-11.6））之间的平均纤毛搏动频率没有差异，但有显著增加（p
Human coronavirus (HCoV) accounts for 15-30% of common colds, but only one case report has described the effect of a coronavirus infection, that was asymptomatic, on human respiratory epithelium.The authors examined the effects of infection with HCoV on ciliary structure and function in healthy volunteers infected by intranasal inoculation with HCoV 229E. A further four volunteers were sham infected with ultraviolet-inactivated virus. Immediately before inoculation (day 0) and 3 days later (day 3), ciliated epithelium was obtained by brushing the inferior nasal turbinate. Ciliary beat frequency was determined and beat pattern analysed for evidence of dyskinesia (0=normal, 3=severely dyskinetic) using digital high-speed video photography. Ciliary ultrastructure was examined by transmission electron microscopy. Symptom diaries were kept for the duration of the study.All subjects inoculated with HCoV, including the three who did not develop symptoms of an upper respiratory tract infection, had disruption of their respiratory epithelium on day 3. Although there was no difference in the mean ciliary beat frequency between day 0 (11.3 Hz (95% confidence interval (CI): 8.6-14.0) and day 3 (9.4 Hz (95% CI 7.2-11.6)), there was a significant increase (p