Transmission of genomic instability from a single irradiated human chromosome to the progeny of unirradiated cells
Transmission of genomic instability from a single irradiated human chromosome to the progeny of unirradiated cells
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DOI：
10.1667/rr0835.1
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发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Watanabe, Masami
中科院分区：
文献类型：
--
作者：
Mukaida, Naoki;Kodama, Seiji;Watanabe, Masami
电离辐射可以诱导染色体不稳定性，这种不稳定性在辐射后在存活细胞的后代中传递许多代，但仍然不清楚为什么这种不稳定性可以传递给后代。为了获得有关基因组不稳定性的传递性的知识，我们通过微细胞融合将辐照的人染色体转移到未辐照的小鼠受体细胞中，并检查了转移的人染色体在微细胞杂交体中的稳定性。转移的染色体是稳定的，在所有六个微细胞杂交，其中一个未经辐照的人类染色体已被引入。相比之下，转移的染色体是不稳定的五个微细胞杂交，其中照射人类染色体已被引入。畸变包括辐射染色体本身的变化和与受体小鼠染色体的重排。因此，本研究表明，基因组不稳定性可以通过暴露于电离辐射的染色体传递给未照射细胞的后代，这意味着不稳定性是由照射的染色体本身引起的，并且不稳定性是由辐射的非靶向效应引起的。(C)2007年，辐射研究学会
Ionizing radiation can induce chromosome instability that is transmitted over many generations after irradiation in the progeny of surviving cells, but it remains unclear why this instability can be transmitted to the progeny. To acquire knowledge about the transmissible nature of genomic instability, we transferred an irradiated human chromosome into unirradiated mouse recipient cells by microcell fusion and examined the stability of the transferred human chromosome in the microcell hybrids. The transferred chromosome was stable in all six microcell hybrids in which an unirradiated human chromosome had been introduced. In contrast, the transferred chromosome was unstable in four out of five microcell hybrids in which an irradiated human chromosome had been introduced. The aberrations included changes in the irradiated chromosome itself and rearrangements with recipient mouse chromosomes. Thus the present study demonstrates that genomic instability can be transmitted to the progeny of unirradiated cells by a chromosome exposed to ionizing radiation, implying that the instability is caused by the irradiated chromosome itself and also that the instability is induced by the nontargeted effect of radiation. (C) 2007 by Radiation Research Society