Crystal structure of plant photosystem I
Crystal structure of plant photosystem I
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DOI：
10.1038/nature02200
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发表时间：
2003-12-11
期刊：
影响因子：
64.8
通讯作者：
Nelson, N
中科院分区：
文献类型：
--
作者：
Ben-Shem, A;Frolow, F;Nelson, N
光合作用是地球上氧气和有机物的主要生产者。将阳光转化为化学能是由两个多亚基膜蛋白复合物（称为光系统I和II）驱动的。我们确定了完整的光系统I（PSI）的晶体结构，从高等植物（豌豆变种）。阿拉斯加）到4.4埃分辨率。其复杂的结构包括12个核心亚基、4种不同的捕光膜蛋白（LHCI）、45个跨膜螺旋、167个叶绿素、3个Fe-S簇和2个叶醌。大约20个叶绿素位于LHCI和核心之间的裂缝中的战略位置。这种结构不仅为探索能量和电子转移提供了一个框架，而且也为探索10亿年前叶绿体从海洋蓝藻中分化出来后形成陆地植物光合装置的进化力量提供了一个框架。
Oxygenic photosynthesis is the principal producer of both oxygen and organic matter on Earth. The conversion of sunlight into chemical energy is driven by two multisubunit membrane protein complexes named photosystem I and II. We determined the crystal structure of the complete photosystem I ( PSI) from a higher plant ( Pisum sativum var. alaska) to 4.4 Angstrom resolution. Its intricate structure shows 12 core subunits, 4 different light- harvesting membrane proteins ( LHCI) assembled in a half- moon shape on one side of the core, 45 transmembrane helices, 167 chlorophylls, 3 Fe - S clusters and 2 phylloquinones. About 20 chlorophylls are positioned in strategic locations in the cleft between LHCI and the core. This structure provides a framework for exploration not only of energy and electron transfer but also of the evolutionary forces that shaped the photosynthetic apparatus of terrestrial plants after the divergence of chloroplasts from marine cyanobacteria one billion years ago.