The CC16 A38G polymorphism is associated with the development of asthma in children with allergic rhinitis
The CC16 A38G polymorphism is associated with the development of asthma in children with allergic rhinitis
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DOI：
10.1111/j.1365-2222.2010.03679.x
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发表时间：
2011-06-01
影响因子：
6.1
通讯作者：
Lue, K. -H.
中科院分区：
文献类型：
--
作者：
Ku, M. -S.;Sun, H. -L.;Lue, K. -H.
虽然哮喘和过敏性鼻炎（AR）被认为是一种综合征，但许多问题仍未得到解答。为什么一些AR患者会发生哮喘，而另一些患者不会，以及哪些因素在哮喘的发展中起作用，迄今尚未清楚阐明。目的：我们假设具有Clara细胞分泌蛋白（CC16，分泌珠蛋白1A1） 38A/38A基因型（rs3741240）的AR患儿患哮喘的可能性增加。方法选取117例急性呼吸道反应（AR）患儿，5年内未确诊为哮喘，作为对照组。病例组（n=202）包括年龄和性别匹配的AR患儿，3-5年后出现哮喘，作为研究组。CC16基因型通过PCR和PCR产物的Sau96I酶切法确定。ELISA法检测血清CC16水平。测定血清总IgE、过敏原特异性IgE、嗜酸性粒细胞计数和肺功能。结果鼻炎合并哮喘患儿AA基因型出现频率显著高于未合并哮喘患儿[优势比(OR)=2.527；95%置信区间(CI)=1.571-4.065；P < 0.01]。患有哮喘的AA基因型鼻炎患儿血清CC16水平显著低于非AA基因型鼻炎患儿和未患哮喘鼻炎患儿。结论及临床意义本研究结果提示CC16 38A/38A基因型在急性鼻炎患儿早期哮喘的发生发展中发挥作用，早期识别鼻炎患儿的危险可能有助于制定哮喘发展的预防措施。
P>BackgroundAlthough asthma and allergic rhinitis (AR) are considered to be one syndrome, many questions remain unanswered. Why do some AR patients develop asthma but others do not, and which factors play a role in the development of asthma that have so far not been clearly elucidated.ObjectiveWe hypothesize that children with AR who have the Clara cell secretory protein (CC16, secretoglobin 1A1) 38A/38A genotype (rs3741240) have an increased likelihood of developing asthma.MethodsThe study sample included 117 children, with AR, but no asthma diagnosed within the following 5 years, as the control group. Cases group (n=202) included age- and gender-matched children with AR first, and asthma developed 3-5 years later, as the study group. The CC16 genotype was determined by PCR and Sau96I restriction digestion of PCR products. The serum CC16 levels were measured by ELISA. Total serum IgE, allergen specific IgE, eosinophil count and pulmonary function were also measured.ResultsIn children with rhinitis who develop asthma, the frequencies of the AA genotype were significantly higher than those who did not develop asthma [odds ratio (OR)=2.527; 95% confidence interval (CI)=1.571-4.065; P < 0.01]. Serum CC16 levels in the children with rhinitis who develop asthma and carry the AA genotype were significantly lower than those who carry the non-AA genotype and those who did not develop asthma.Conclusions and Clinical RelevanceResults of this study suggest that CC16 38A/38A genotype plays a role in the development of early asthma in children with AR. Early identification of rhinitis children at risk may assist in designing preventative approach to asthma development.