CORTICAL FINE ACTIN-FILAMENTS IN HIGHER-PLANT CELLS VISUALIZED BY RHODAMINE-PHALLOIDIN AFTER PRETREATMENT WITH M-MALEIMIDOBENZOYL N-HYDROXYSUCCINIMIDE ESTER
CORTICAL FINE ACTIN-FILAMENTS IN HIGHER-PLANT CELLS VISUALIZED BY RHODAMINE-PHALLOIDIN AFTER PRETREATMENT WITH M-MALEIMIDOBENZOYL N-HYDROXYSUCCINIMIDE ESTER
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DOI：
10.1007/bf02079325
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发表时间：
1989-01-01
期刊：
影响因子：
2.9
通讯作者：
SHIBAOKA, H
中科院分区：
文献类型：
--
作者：
SONOBE, S;SHIBAOKA, H
用100微米间马来苯甲酰基N-羟基琥珀酰亚胺酯(MBS)预处理后，用罗丹明-鬼笔环素对培养的烟草细胞肌动蛋白细丝进行染色。在甲醛固定之前使用MBS使我们能够在大多数间期烟草细胞中看到细小的、横向排列的皮质肌动蛋白细丝。它还使我们能够在相同的细胞中对细小的肌动蛋白细丝和微管进行双重染色。单个烟草细胞皮质区的肌动蛋白细丝和微管的图案彼此非常相似。使用MBS的方法也被发现在显示洋葱鳞茎内表皮细胞中的细小皮质肌动蛋白细丝方面是有用的。当罗丹明-鬼臼毒素应用于未固定的细胞时，似乎诱导了烟草细胞和洋葱细胞中肌动蛋白细丝的捆绑，这表明将荧光染料标记的鬼臼毒素应用于未固定的细胞存在观察到人工捆绑的肌动蛋白细丝的风险。
Actin filaments in cultured tobacco cells were stained by rhodamine-phalloidin after pretreatment with 100 .mu.M m-maleidobenzoyl N-hydroxysuccinimide ester (MBS) followed by formaldehyde fixation. The use of MBS prior to formaldehyde fixation enabled us to visualize fine, transversely arranged cortical actin filaments in a majority of interphase tobacco cells. It also enabled us to double-stain fine actin filaments and microtubules in the same cells. The pattern of actin filaments and that of microtubules in the cortical region of a single tobacco cell bore a close resemblance to each other. The method which employed MBS was found to be useful also in visualizing fine cortical actin filaments in inner epidermal cells of onion bulbs. Rhodamine-phalloidin seemed to induce the bundling of actin filaments both tobacco cells and in onion cells when it was applied to the cells which had not been subjected to fixation, indicating that the application of fluorescent-dye-labeled phallotoxins to unfixed cells involves the risk of observing artificially bundled actin filaments.