Metabolism of 7,12-dimethylbenz[a]anthracene by mouse mammary epithelial cells cultured serum-free inside collagen gels.
Metabolism of 7,12-dimethylbenz[a]anthracene by mouse mammary epithelial cells cultured serum-free inside collagen gels.
复制标题
胶原凝胶内无血清培养的小鼠乳腺上皮细胞对 7,12-二甲基苯并[a]蒽的代谢。
DOI：
10.1016/0304-3835(88)90002-x
复制
发表时间：
1988
期刊：
影响因子：
9.7
通讯作者：
Nandi,S
中科院分区：
文献类型：
--
作者：
Guzman,RC;Osborn,RC;Bartley,JC;Nandi,S
摘要将3-4月龄的BALB B c处女小鼠的乳腺上皮细胞在胶原凝胶内培养在以下无血清培养基中：Dulbecco改良Eagle培养基/Ham F-12（1：1），补充有（A）胰岛素、牛血清白蛋白、表皮生长因子;（B）胰岛素、牛血清白蛋白、孕酮、催乳素;（C）胰岛素、牛血清白蛋白、孕酮、催乳素、亚油酸。细胞数量随着所用培养基的增加而增加。在培养的第7天，将[3 H]二甲基苯并[a]蒽（DMBA）加入到培养物中，并测定其代谢为水溶性和有机可溶性化合物。小鼠乳腺上皮细胞能够将[3 H] DMBA代谢为水和有机可溶性代谢物。到72小时，77-94%的添加的DMBA已被代谢的上皮细胞在三种介质中的水和有机可溶性代谢物的等量。水溶性和有机溶性代谢物之间的分布大致相等。来自培养基的有机可溶性级分的高压液相色谱图表明，主要产物似乎是酚类、2-、3-或4-羟基二甲基苯并[a]蒽、羟甲基衍生物、7-甲基苯并[a]蒽和7-羟甲基苯并[a]蒽、反式-3，4-二氢-3，4-二氢苯并[a]蒽，4-二羟基二甲基苯并[a]蒽和刚好在标记物顺式-5，6-二氢-5，6-二羟基二甲基苯并[a]蒽之后洗脱的一个或两个主要级分。刚好在顺式-5，6-二氢-5，6-二羟基二甲基苯并[a]蒽之前洗脱的主要级分很可能是反式-8，9-二氢8，9-二羟基二甲基苯并[a]蒽.在所有三种无血清培养基中培养的细胞的特征相似。结果表明，小鼠乳腺上皮细胞内培养的胶原凝胶与无血清培养基可以代谢DMBA推定致癌形式。
Abstract Mammary epithelial cells from 3–4-month-old BALB c virgin mice were cultured inside collagen gels in the following serum-free media: Dulbecco's Modified Eagle's Medium/Ham's F-12 (1: 1) supplemented with (A) insulin, bovine serum albumin, epidermal growth factor;(B) insulin, bovine serum albumin, progesterone, prolactin;(C) insulin, bovine serum albumin, progesterone, prolactin, linoleic acid. Cell number increased with all media used. At day 7 of culture,[3 H] dimethylbenz [a] anthracene (DMBA) was added to the cultures and its metabolism to water soluble and organic soluble compounds was determined. Mouse mammary epithelial cells were able to metabolize [3 H] DMBA to water and organosoluble metabolites. By 72 h, 77–94% of the added DMBA had been metabolized by the epithelial cells in the three media to water and organosoluble metabolites in equivalent amounts. The distribution between water soluble and organosoluble metabolites was approximately equivalent. The high pressure liquid chromatography profiles of organosoluble fractions from the media indicated that the major products appeared to be the phenols, 2-, 3-, or 4-hydroxydimethylbenz [a] anthracene, the hydroxymethyl derivatives, 7-methylbenz [a] anthracene and 7-hydroxymethylbenz [a] anthracene, trans-3, 4-dihydro-3, 4-dihydroxydimethylbenz [a] anthracene and one or two major fractions eluting just behind the marker cis-5, 6-dihydro-5, 6-dihydroxydimethylbenz [a] anthracene. The major fraction eluting just ahead of the cis-5, 6-dihydro-5, 6-dihydroxydimethylbenz [a] anthracene was most likely trans-8, 9-dihydro 8, 9-dihydroxydimethylbenz [a] anthracene. The profiles were similar for the cells cultured in all three serum-free media. The results demonstrate that mouse mammary epithelial cells cultured inside collagen gels with serum-free media can metabolize DMBA to putative carcinogenic forms.