Thymidine phosphorylase expression in normal, hyperplastic and neoplastic prostates: correlation with tumour associated macrophages, infiltrating lymphocytes, and angiogenesis.
Thymidine phosphorylase expression in normal, hyperplastic and neoplastic prostates: correlation with tumour associated macrophages, infiltrating lymphocytes, and angiogenesis.
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DOI：
10.1038/sj.bjc.6600281
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发表时间：
2002-05-06
影响因子：
8.8
通讯作者：
Koukourakis MI
中科院分区：
文献类型：
--
作者：
Sivridis E;Giatromanolaki A;Papadopoulos I;Gatter KC;Harris AL;Koukourakis MI
胸苷磷酸化酶是一种血管生成因子，主要由许多人类恶性肿瘤中的癌细胞、基质细胞和肿瘤相关巨噬细胞表达。这些不同类型的胸苷磷酸化酶表达细胞，然而，可能有一个独特的地方在血管生成过程中，这个问题是解决在本研究中。应用胸苷磷酸化酶（P-GF. 44 C）、肿瘤相关巨噬细胞（CD 68）、内皮细胞（CD 31）和前列腺特异性抗原（ER-PR 8）的特异性抗体，对20例正常/增生前列腺和60例前列腺癌进行了免疫组化研究。胸苷磷酸化酶表达的正常和增生的上皮细胞或基质细胞几乎完全发生在一个激烈的淋巴细胞浸润的背景下。高胸苷磷酸化酶癌细胞和胸苷磷酸化酶基质细胞表达与前列腺癌中的高血管生成相关，并且这种显著的关联扩展到包括肿瘤相关巨噬细胞和肿瘤浸润淋巴细胞。胸苷磷酸化酶表达和肿瘤浸润淋巴细胞与前列腺特异性抗原反应性呈负相关。总之，胸苷磷酸化酶是前列腺癌中的主要血管生成因子，其上调可能发生在宿主免疫应答的背景下。英国癌症杂志（2002）86，1465-1471。DOI：10.1038/sj/bjc/6600281 www.bjcancer.com © 2002英国癌症研究中心
Thymidine phosphorylase is an angiogenic factor primarily expressed by cancer cells, stromal cells and tumour-associated macrophages in many human malignancies. These different types of thymidine phosphorylase-expressing cells, however, may have a distinct place in the angiogenic process, and this question was addressed in the present study. A series of 20 normal/hyperplastic prostate glands and 60 prostate carcinomas was investigated by immunohistochemistry, using specific antibodies for thymidine phosphorylase (P-GF.44C), tumour-associated macrophages (CD68), endothelium (CD31) and prostate specific antigen (ER-PR8). Thymidine phosphorylase expression by normal and hyperplastic epithelial or stromal cells occurred almost exclusively in the context of an intense lymphocytic infiltrate. High thymidine phosphorylase cancer cells and thymidine phosphorylase stromal cells expression was associated with high angiogenesis in prostate carcinomas, and this significant association was extended to include both tumour-associated macrophages and tumour-infiltrating lymphocytes. Thymidine phosphorylase expression and tumour-infiltrating lymphocytes were related inversely with prostate specific antigen reactivity. In conclusion, thymidine phosphorylase is a major angiogenic factor in prostate carcinomas and its up-regulation is likely to occur in the context of a host immune response. British Journal of Cancer (2002) 86, 1465–1471. DOI: 10.1038/sj/bjc/6600281 www.bjcancer.com © 2002 Cancer Research UK