A rapid and specific ultramicro method for total serum cholesterol.
A rapid and specific ultramicro method for total serum cholesterol.
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一种快速、特异的血清总胆固醇超微量方法。
DOI：
10.1093/clinchem/8.3.302
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发表时间：
1962
影响因子：
9.3
通讯作者：
Richard C. Wolf
中科院分区：
文献类型：
--
作者：
Robert E. Bowman;Richard C. Wolf
介绍了Zak胆固醇反应的一种改进方法，即用乙醇取代冰醋酸。这种修饰避免了净化冰醋酸的危险，并通过消除溴化物或碘盐的干扰增加了反应的特异性。乙醇反应具有通常的醋酸反应的吸收光谱、灵敏度和稳定性。
基于这一乙醇反应，建立了一种快速的超微量方法。一个0.01到0.05毫升。血清样品用2.5毫升乙醇和2.0毫升乙醇提取。该提取物的等量与2.0毫升的ZAK显色剂直接反应。用该程序和Sperry-Webb方法测定的胆固醇浓度在猴子和人的血清中显示出令人满意的一致性，包括正常的、溶血的、高胆固醇的和严重的高脂血症或黄疸的血清。
A modification of the Zak cholesterol reaction is described in which ethanol is substituted for glacial acetic acid. This modification avoids the hazard of purifying glacial acetic acid and also increases the specificity of the reaction by eliminating interference from bromide or iodide salts. The ethanolic reaction has the absorbance spectrum, sensitivity, and stability of the usual acetic acid reaction.
A rapid ultramicro method has been developed based on this ethanolic reaction. A 0.01- to 0.05-ml. sample of serum is extracted with 2.5 ml. of ethanol, and a 2.0-ml. aliquot of this extract is reacted directly with 2.0 ml. of a Zak color reagent. Cholesterol concentrations as determined by this procedure and by the Sperry-Webb method showed satisfactory agreement for monkey and human sera, including normal, hemolyzed, high cholesterol, and severely lipemic or jaundiced sera..