Asthma pregnancy alters postnatal development of chromaffin cells in the rat adrenal medulla.
Asthma pregnancy alters postnatal development of chromaffin cells in the rat adrenal medulla.
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哮喘妊娠改变大鼠肾上腺髓质中嗜铬细胞的产后发育
DOI：
10.1371/journal.pone.0020337
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Feng JT
中科院分区：
文献类型：
--
作者：
Wu XM;Hu CP;Li XZ;Zou YQ;Zou JT;Li YY;Feng JT
背景肾上腺神经内分泌在哮喘发病中起重要作用。交感肾上腺系统的活动可以被早期生活事件改变。妊娠期母亲哮喘对后代肾上腺髓质的影响尚不清楚。方法/主要发现本研究的目的是探讨母亲在怀孕期间哮喘对子代出生后3天（P3）至出生后60天（P60）肾上腺髓质发育和功能的影响。用卵白蛋白（OVA）致敏哮喘孕鼠（AP）、神经生长因子（NGF）处理孕鼠（NP）和NGF抗体处理孕鼠（ANP），分别用NGF和NGF抗体处理NP和ANP。将母体组的子代大鼠分为4组：对照妊娠大鼠（OCP）的子代、AP（OAP）的子代、NP（ONP）的子代和ANP（OANP）的子代。分析肾上腺髓质苯乙醇胺N-甲基转移酶（PNMT）蛋白的表达。测定血清肾上腺素（EPI）、皮质酮和神经生长因子（NGF）的浓度。肾上腺髓质嗜铬细胞（AMCC）在OAP和ONP中易于分化为交感神经细胞。OAP组EPI和PNMT在P3 ~ P14降低，P30 ~ P60逐渐恢复正常，ONP组从出生到成年均较低。皮质酮浓度在OAP和ONP中显著增加。结论妊娠期哮喘可促进AMCC向交感神经元分化，抑制EPI合成，导致支气管舒张功能障碍。
Background Adrenal neuroendocrine plays an important role in asthma. The activity of the sympathoadrenal system could be altered by early life events. The effects of maternal asthma during pregnancy on the adrenal medulla of offspring remain unknown. Methodology/Principal Findings This study aims to explore the influence of maternal asthma during pregnancy on the development and function of adrenal medulla in offspring from postnatal day 3 (P3) to postnatal day 60 (P60). Asthmatic pregnant rats (AP), nerve growth factor (NGF)-treated pregnant rats (NP) and NGF antibody-treated pregnant rats (ANP) were sensitized and challenged with ovalbumin (OVA); NP and ANP were treated with NGF and NGF antibody respectively. Offspring rats from the maternal group were divided into four groups: offspring from control pregnant rats (OCP), offspring from AP (OAP), offspring from NP (ONP), and offspring from ANP (OANP). The expressions of phenylethanolamine N-methyltransferase (PNMT) protein in adrenal medulla were analyzed. The concentrations of epinephrine (EPI), corticosterone and NGF in serum were measured. Adrenal medulla chromaffin cells (AMCC) were prone to differentiate into sympathetic nerve cells in OAP and ONP. Both EPI and PNMT were decreased in OAP from P3 to P14, and then reached normal level gradually from P30 to P60, which were lower from birth to adulthood in ONP. Corticosterone concentration increased significantly in OAP and ONP. Conclusion/Significance Asthma pregnancy may promote AMCC to differentiate into sympathetic neurons in offspring rats and inhibit the synthesis of EPI, resulting in dysfunction of bronchial relaxation.
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DOI：
10.1002/jemt.1060120412
发表时间：
1989-08-01
期刊：
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE
影响因子：
--
作者：
HOFMANN, HD;SEIDL, K;UNSICKER, K
通讯作者：
UNSICKER, K
影响因子：
4.1
作者：
Molendi-Coste, Olivier;Laborie, Christine;Breton, Christophe
通讯作者：
Breton, Christophe
影响因子：
5.5
作者：
Carabelli, V.;Marcantoni, A.;Carbone, E.
通讯作者：
Carbone, E.
影响因子：
4.7
作者：
Feng, JT;Hu, CP
通讯作者：
Hu, CP
DOI：
10.1152/ajplung.00414.2001
发表时间：
2002-08-01
影响因子：
4.9
作者：
Hu, CP;Wedde-Beer, K;Piedimonte, G
通讯作者：
Piedimonte, G