Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.
Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.
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DOI：
10.1107/s0907444998008130
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
H. Hamana;H. Moriyama;T. Shinozawa;N. Tanaka
中科院分区：
文献类型：
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作者：
H. Hamana;H. Moriyama;T. Shinozawa;N. Tanaka
采用悬滴气相扩散法结合大孔种子发酵剂，实现了嗜热嗜热菌(Thermus Thermophilus)轮转酸磷酸核糖转移酶的结晶。在2-甲基-2，4-戊二醇存在下，在298K或更低的温度下，在新鲜的蛋白质溶液中生长出针状的小微晶。虽然这些常温条件导致了堆积结晶，但温度升高到310K时允许晶体生长。这是由于在较高温度下酶的溶解度增加所致。晶体属单斜晶系P21，晶胞参数a=44.4，b=59.6，c=67.8A，β=98.3度。
Crystallization of orotate phosphoribosyltransferase from a thermophilic organism, Thermus thermophilus, was achieved using the hanging-drop vapour-diffusion method coupled with a macroseeding starter. Small needle-like microcrystals were grown in a fresh protein solution in the presence of 2-methyl-2,4-pentanediol at 298 K or below. Although these normal temperature conditions caused stacking crystallization, an increase of temperature to 310 K permitted crystal growth. This was because of increased enzyme solubility at the higher temperature. The crystal was found to belong to the monoclinic space group P21with unit-cell parameters a = 44.4, b = 59.6, c = 67.8 A and beta = 98.3 degrees.