THE DEVELOPMENT OF THE SINUSOIDS OF FETAL RAT-LIVER - MORPHOLOGY OF ENDOTHELIAL-CELLS, KUPFFER CELLS, AND THE TRANSMURAL MIGRATION OF BLOOD-CELLS INTO THE SINUSOIDS
THE DEVELOPMENT OF THE SINUSOIDS OF FETAL RAT-LIVER - MORPHOLOGY OF ENDOTHELIAL-CELLS, KUPFFER CELLS, AND THE TRANSMURAL MIGRATION OF BLOOD-CELLS INTO THE SINUSOIDS
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DOI：
10.1002/aja.1001590102
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发表时间：
1980-01-01
影响因子：
--
通讯作者：
PINO, RM
中科院分区：
文献类型：
--
作者：
BANKSTON, PW;PINO, RM
本文研究了孕10 ~ 22天大鼠胎肝血窦精细结构的发育。通过脐静脉将胶体碳（Pelikan墨水）注射到妊娠14-22天的胎儿中，以评估窦状隙衬里的连续性和发育衬里细胞的吞噬能力。内皮细胞，缺乏底层的基底膜，在所有胎龄形成大部分的血管衬里。这些细胞在孕17天前具有典型的细胞间连接和有隔膜的窗孔。典型的成人肝脏的经内皮开放性开窗，出现在妊娠17天左右，在妊娠的剩余时间内数量增加。虽然在妊娠16天之前，具有隔膜的开窗可渗透碳，但在妊娠17天首次观察到的开放开窗允许大量碳到达血管外空间。内皮细胞对碳的内吞作用完全由大的刷毛包被的囊泡完成。内皮细胞还参与了血管外空间新形成的红细胞和巨核细胞突起的透壁渗出，形成了一个暂时的迁移孔，允许这些细胞和突起进入循环。在妊娠末期，造血活动几乎停止，只有Disse间隙将衬里细胞与实质细胞分开。库普弗细胞很容易识别，早在13天的妊娠内容物的吞噬体和吞噬的红细胞。枯否细胞比内皮细胞更热衷于吞噬碳。在妊娠末期，一些枯否细胞形成了一层均匀的碳粘衣。
The fine structural development of rat fetal liver sinusoids from 10 to 22 days gestation was studied. Colloidal carbon (Pelikan ink) was injected into 14-22 day gestation fetuses via the umbilical vein to assess the continuity of the sinusoidal lining and the phagocytic ability of the developing lining cells. Endothelial cells, devoid of an underlying basal lamina, form the bulk of the vascular lining at all gestational ages. These cells possess typical intercellular junctions and fenestrae with diaphragms before 17 days gestation. Transendothelial open fenestrations, typical of the adult liver, appear around 17 days gestation, increasing in number of the remainder of gestation. Although fenestrae possessing diaphragms are permeable to carbon before 16 days gestation, open fenestrations, first seen at 17 days gestation, allowed large amounts of carbon to reach the extravascular space. Endocytosis of carbon by endothelial cells was accomplished exclusively by large bristle-coated vesicles. Endothelial cells were also involved in transmural diapedesis of newly formed erythrocytes and megakaryocyte processes from the extravascular space by forming a temporary migration pore allowing these cells and processes to enter the circulation. At the end of gestation, blood-forming activity nearly ceased, and only the space of Disse separated the lining cells from the parenchymal cells. Kupffer cells were easily identified as early as 13 days gestation by their content of phagosomes and engulfed erythrocytes. Kupffer cells are much more avid in the phagocytosis of carbon than are endothelial cells. Toward the end of gestation, some Kupffer cells develop a homogeneous sticky coat to carbon.