A promoter element that exerts positive and negative control of the interleukin 2-responsive J-chain gene.
A promoter element that exerts positive and negative control of the interleukin 2-responsive J-chain gene.
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对白细胞介素 2 反应性 J 链基因发挥正向和负向控制的启动子元件。
DOI：
10.1073/pnas.89.13.5966
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发表时间：
1992
影响因子：
11.1
通讯作者：
Koshland,ME
中科院分区：
文献类型：
--
作者：
Lansford,RD;McFadden,HJ;Siu,ST;Cox,JS;Cann,GM;Koshland,ME
在初级免疫反应中，来自白介素2(IL-2)的信号诱导B淋巴细胞表达与IgM连接的成分J链的基因。本研究通过检测J链基因5‘侧翼区的调控序列和相互作用的核因子来探讨其信号转导机制。分析确定了一个位于-75到-45之间的主控区域，它编码两个相邻的元件：一个富含T的序列(JA)，包含一个单一的正调控基序；以及一个富含A+G的序列(JB)，包含重叠的正负调控基序。对这两个元件的分析表明，双功能JB序列可能是IL-2信号的靶标。这一证据基于以下发现：(I)JB活性与J链基因转录相关--即JB在J链沉默的B细胞中作为抑制因子，在J链表达的细胞中作为激活剂，以及(Ii)JB的激活功能是由B细胞特有的核蛋白NF-JB介导的，它呈现出IL-2反应的结合模式。
In a primary immune response a signal from interleukin 2 (IL-2) induces B lymphocytes to express the gene for the IgM joining component, the J chain. The signaling mechanism was pursued in this study by examining the J-chain gene 5' flanking region for regulatory sequences and interacting nuclear factors. The analyses identified a major control region located between -75 and -45 that encodes two adjacent elements: a T-rich sequence (JA) containing a single positive regulatory motif and an A+G-rich sequence (JB) containing overlapping positive and negative regulatory motifs. Dissection of the two elements indicated that the bifunctional JB sequence is the likely target of the IL-2 signal. The evidence was based on findings that (i) JB activity correlated with J-chain gene transcription--i.e., JB acts as a repressor in J-chain-silent B cells and as an activator in J-chain-expressing cells, and (ii) JB activator function is mediated by a B-cell-specific nuclear protein, NF-JB, that exhibits an IL-2-responsive binding pattern.