A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms
A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms
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DOI：
10.1002/elps.201700039
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发表时间：
2017-10
期刊：
影响因子：
2.9
通讯作者：
Naoaki Okura;Yuta Nakashoji;Toshihiro Koshirogane;M. Kondo;Y. Tanaka;Kohei Inoue;M. Hashimoto
中科院分区：
文献类型：
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作者：
Naoaki Okura;Yuta Nakashoji;Toshihiro Koshirogane;M. Kondo;Y. Tanaka;Kohei Inoue;M. Hashimoto
我们开发了一种紧凑、方便的微流控液滴产生装置，该装置由具有T形通道结构的聚二甲基硅氧烷微流控芯片、两个入口储液器和一个出口储液器和一个带控制器的压电式(PZT)隔膜微泵组成。使用PZT泵将空气从出口储气罐中排出，从而降低了储气罐内的压力。出口储液器内的压力降低将进口储液器中预装的油和水溶液拉入微通道，然后在T形连接处汇合，以每秒∼1000的速度成功地形成了油包水乳状液，样品损失最小。我们证实，液滴的形成是在打开泵后立即发生的(<；1 S)。通过重复运行，液滴的形成具有高度的重复性，液滴大小纯度(多分散性，<；4%)与使用其他微流控液滴制备技术所获得的液滴纯度相当。我们还在创建的液滴中演示了单分子PCR扩增，这表明该设备可以用于大多数实验室的有效液滴数字聚合酶链式反应平台，而不需要花费大量资金、空间或时间来获取技术技能。
We have exploited a compact and facile microfluidic droplet creation device consisting of a poly(dimethylsiloxane) microfluidic chip possessing T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir, and a piezoelectric (PZT) diaphragm micropump with controller. Air was evacuated from the outlet reservoir using the PZT pump, reducing the pressure inside. The reduced pressure within the outlet reservoir pulled oil and aqueous solution preloaded in the inlet reservoirs into the microchannels, which then merged at the T‐junction, successfully forming water‐in‐oil emulsion droplets at a rate of ∼1000 per second with minimal sample loss. We confirmed that the onset of droplet formation occurred immediately after turning on the pump (<1 s). Over repeated runs, droplet formation was highly reproducible, with droplet size purity (polydispersity, <4%) comparable to that achieved using other microfluidic droplet preparation techniques. We also demonstrated single‐molecule PCR amplification in the created droplets, suggesting that the device could be used for effective droplet digital PCR platforms in most laboratories without requiring great expense, space, or time for acquiring technical skills.